Cathepsin H Inhibitor Assay Kit (Fluorometric) (ab211080)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Inhibitor compounds
Overview
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Product name
Cathepsin H Inhibitor Assay Kit (Fluorometric)
See all Cathepsin H kits -
Detection method
Fluorescent -
Sample type
Inhibitor compounds -
Assay type
Quantitative -
Product overview
Cathepsin H Inhibitor Screening Kit (Fluorometric) (ab211080) provides a rapid, simple, sensitive and reliable test suitable for high-throughput screening of Cathepsin H inhibitors. It utilizes the ability of an active Cathepsin H to cleave a synthetic AFC-based peptide substrate to release free AFC, which can be easily quantified using a fluorometer or fluorescence microplate reader. In the presence of a Cathepsin H-specific inhibitor, the cleavage of this substrate is reduced/abolished resulting in decrease or total loss of the AFC fluorescence.
This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of Cathepsin H.
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Notes
Cathepsin H (CTSH, EC 3.4.22.16), a lysosomal cysteine protease, is important in the overall degradation of lysosomal proteins. It is composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. The encoded protein, which belongs to the peptidase C1 protein family, can act both as an aminopeptidase and as an endopeptidase. Increased expression of this gene has been correlated with malignant progression of prostate tumors.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests CTSH Assay Buffer 1 x 25ml CTSH Inhibitor (1 mM in DMSO) 1 x 20µl CTSH Reagent 1 x 100µl CTSH Substrate (in DMSO) 1 x 200µl Human Cathepsin H (5 µg) 1 vial -
Research areas
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Relevance
The cathepsin family of proteolytic enzymes contains several diverse classes of proteases. The cysteine protease class comprises cathepsins B, L, H, K, S, and O. The aspartyl protease class is composed of cathepsins D and E. Cathepsin G is in the serine protease class. Most cathepsins are lysosomal and each is involved in various cellular events such as peptide biosynthesis and protein degradation. The propeptide region of cathepsin H is highly homologous to other cathepsins. This region is thought to play a role in the processing of cysteine proteases. Cathepsin H is thought to be involved in several pathological conditions, including neuro degenerative disorders and cancer. -
Cellular localization
Lysosome -
Alternative names
- ACC 4
- ACC 5
- ACC4
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Images
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Cathepsin H Inhibitor Screening Kit (Fluorometric) (ab211080)
Typical inhibition curve of Cathepsin H by Cathepsin H inhibitor.
