Anti-KMT1A / SUV39H1 antibody [44.1] (ab12405)
![Anti-KMT1A / SUV39H1 antibody [44.1] (ab12405)](https://www.abcam.com/ps/products/12/ab12405/Images/ab12405-308631-anti-kmt1a-suv39h1-antibody-441-chip-grade-western-blot.jpg "Anti-KMT1A / SUV39H1 antibody [44.1] (ab12405)")
Key features and details
- Mouse monoclonal [44.1] to KMT1A / SUV39H1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-KMT1A / SUV39H1 antibody [44.1]
See all KMT1A / SUV39H1 primary antibodies -
Description
Mouse monoclonal [44.1] to KMT1A / SUV39H1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
corresponding to KMT1A/ SUV39H1.
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Epitope
Ab 12405 recognizes an epitope in the N-terminal (195 amino acids) of human and mouse SUV39H1 Histone Methyltransferase. -
Positive control
- HCT116, U87-MG and TE671 cell lysates.
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General notes
This antibody clone is manufactured by Abcam.
Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
44.1 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-KMT1A / SUV39H1 antibody [44.1] (ab12405)All lanes : Anti-KMT1A / SUV39H1 antibody [44.1] (ab12405) at 5 µg
Lane 1 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
Lane 2 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
Lane 3 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Additional bands at: 36 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab12405 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
