Anti-LDL Receptor antibody [032] - BSA and Azide free (ab277134)
![Anti-LDL Receptor antibody [032] - BSA and Azide free (ab277134)](https://www.abcam.com/ps/products/277/ab277134/Images/ab277134-2-AntiLDL-Receptor-antibody-Flow-cyt-Raw2647.jpg "Anti-LDL Receptor antibody [032] - BSA and Azide free (ab277134)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [032] to LDL Receptor - BSA and Azide free
- Suitable for: Flow Cyt, ICC
- Reacts with: Mouse
Overview
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Product name
Anti-LDL Receptor antibody [032] - BSA and Azide free
See all LDL Receptor primary antibodies -
Description
Rabbit monoclonal [032] to LDL Receptor - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseICC Mouse
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Immunogen
Recombinant fragment corresponding to Mouse LDL Receptor aa 1-790. Genbank: NP_034830.2
Database link: P35951 -
Positive control
- Flow cyt: Raw264.7 cells. ICC: Raw264.7 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS
0.2 µm filtered solution -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
032 -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Images
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Flow Cytometry - Anti-LDL Receptor antibody (ab277134)
Flow cytometric analysis of LDL Receptor expression on Raw264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells. The cells were stained with ab277134 at 1/25 dilution, then a FITC-conjugated second step antibody (Black). Cells were also stained with an isotype control (Grey). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
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Immunocytochemistry - Anti-LDL Receptor antibody (ab277134)Immunofluorescence staining of LDL Receptor in Raw264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with ab277134 at 1/100 at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope) Goat Anti-rabbit IgG secondary antibody(green), counterstained with DAPI (blue).
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![Anti-LDL Receptor antibody [032] - BSA and Azide free (ab277134)](https://www.abcam.com/ps/products/277/ab277134/Images/ab277134-3-benefits-of-recombinant-antibodies.png)
Anti-LDL Receptor antibody [032] - BSA and Azide free (ab277134)
