Plasma Membrane Protein Extraction Kit (ab65400)
Key features and details
- Assay time: 1 hr
Overview
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Product name
Plasma Membrane Protein Extraction Kit -
Assay time
1h 00m -
Product overview
Plasma Membrane Protein Extraction Kit ab65400 provides optimized buffers and reagents for effective extraction of plasma membrane proteins from mammalian tissues and cells.
Other commercially available methods can only extract the total cellular membrane proteins (combinations of plasma and organelle membrane proteins). The ab65400 kit offers two options: either extract the total cellular membrane proteins, or purify the plasma membrane proteins specifically.
The procedure offers consistent yield and high purity (over 90%). Membrane proteins prepared using the kit can be utilized in a variety of applications, such as Western blotting, 2-D gels, and enzyme analyses, etc. The entire procedure takes less than 1 hour.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 50 tests Homogenize Buffer NM 1 x 100ml Lower Phase Solution WM 1 x 20ml Protease Inhibitor Cocktail (Lyophilised) Red 1 vial Upper Phase Solution NM 1 x 20ml -
Research areas
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Relevance
Membrane Protein Extraction is a method for extraction of membrane proteins from mammalian tissues and cells. Many procedures extract total cellular membrane proteins which is a combination of plasma and organelle membrane proteins.
Images
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Functional Studies - Plasma Membrane Protein Extraction Kit (ab65400) Kweon, Hae-Jin et al., Scientific reports?vol. 6 30684., Fig 1, doi:10.1038/srep30684Western blotting on the plasma membrane (PM) fraction extracted useing ab65400, total cellular membrane (TCM) fraction, and total lysate of cells expressing GFP-tagged ASIC2a or ASIC2b was performed using anti-GFP antibody. As controls, the PM and the TCM fractions were blotted using anti-calnexin (CNX) antibody, and total lysate was blotted using anti-GAPDH antibody.
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Membrane protein assay - ab65400 Image from Choveau F.S et al., PLoS One 10(12), Fig 2C. Doi: 10.1371/journal.pone.0145367. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Cell membrane protein was collected using plasma membrane protein extraction kit (ab65400). The upper panels show the plasma membrane myc-tagged protein; the middle panels show the cytosolic myc-tagged proteins and the lower panels show the total myc-tagged protein from CHO (Chinese Hamster ovary) whole-cell lysates.