Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Smad2 antibody [7A5] (ab71109)

Key features and details

  • Mouse monoclonal [7A5] to Smad2
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-Smad2 antibody [7A5]
    See all Smad2 primary antibodies

  • Description

    Mouse monoclonal [7A5] to Smad2

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length human Smad2 protein purified from E.coli with His tag.

  • Positive control

    • HeLa, A431, NIH3T3 and PC12 cell lysates.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 18th September 2017. Lot numbers higher than GR183929 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Flow Cytometry - Anti-Smad2 antibody [7A5] (ab71109)
    Flow Cytometry - Anti-Smad2 antibody [7A5] (ab71109)
    Overlay histogram showing PC3 cells stained with ab71109 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab71109, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Western blot - Anti-Smad2 antibody [7A5] (ab71109)
    Western blot - Anti-Smad2 antibody [7A5] (ab71109)
    All lanes : Anti-Smad2 antibody [7A5] (ab71109) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : NIH3T3 cell lysate
    Lane 4 : PC12 cell lysate

    Predicted band size: 58 kDa
    Observed band size: 58 kDa

  • Western blot - Anti-Smad2 antibody [7A5] (ab71109)
    Western blot - Anti-Smad2 antibody [7A5] (ab71109)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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