Anti-n-Myc/MYCN antibody [EPR18982-13] (ab189528)
![Anti-n-Myc/MYCN antibody [EPR18982-13] (ab189528)](https://www.abcam.com/ps/products/189/ab189528/Images/ab189528-260313-anti-n-myc-antibody-epr18982-13-western-blot.jpg "Anti-n-Myc/MYCN antibody [EPR18982-13] (ab189528)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18982-13] to n-Myc/MYCN
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-n-Myc/MYCN antibody [EPR18982-13]
See all n-Myc/MYCN primary antibodies -
Description
Rabbit monoclonal [EPR18982-13] to n-Myc/MYCN -
Host species
Rabbit -
Specificity
Detection of endogenous expression of n-Myc/MYCN in WB will need optimization. Under our experimental conditions we could not detect endogenous n-Myc/MYCN in human cell lysates.
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Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293 whole cell lysate transfected with n-Myc/MYCN with GFP-tag.
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General notes
This product was previously labelled as n-Myc
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18982-13 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-n-Myc/MYCN antibody [EPR18982-13] (ab189528)All lanes : Anti-n-Myc/MYCN antibody [EPR18982-13] (ab189528) at 1/1000 dilution
Lanes 1 & 3 & 5 : Untransfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate (control)
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with n-Myc/MYCN with GFP-tag
Lane 4 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with l-Myc with GST-tag
Lane 6 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with c-Myc with His-tag
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Additional bands at: 83 kDa (possible tagged protein)
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
n-Myc/MYCN is tagged with GFP, therefore its expected MW is 83 kD under these conditions.
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![Immunoprecipitation - Anti-n-Myc/MYCN antibody [EPR18982-13] (ab189528)](https://www.abcam.com/ps/products/189/ab189528/Images/ab189528-269313-anti-n-myc-antibody-epr18982-13-immunoprecipitation.jpg)
Immunoprecipitation - Anti-n-Myc/MYCN antibody [EPR18982-13] (ab189528)n-Myc/MYCN was immunoprecipitated from 2μg of HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysates with ab194094 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab189528 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HEK-293 whole cell lysate transfected with GFP-tagged n-Myc/MYCN construct, 10ug (Input).
Lane 2: ab189528 IP in HEK-293 whole cell lysate transfected with GFP-tagged n-Myc/MYCN construct.
Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab189528 IP in HEK-293 whole cell lysate transfected with GFP-tagged n-Myc/MYCN construct.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
ab189528 immuno-precipitated the overexpressed n-Myc/MYCN-GFP (~100 kDa) in the transfected HEK-293 lysate and an additional 50 kDa off-target band. The overexpressed n-Myc/MYCN-GFP was recognized by ab290 (anti-GFP antibody, IP #2) and ab16898 (anti-n-Myc/MYCN antibody, IP #3).
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![Immunoprecipitation - Anti-n-Myc/MYCN antibody [EPR18982-13] (ab189528)](https://www.abcam.com/ps/products/189/ab189528/Images/ab189528-269314-anti-n-myc-antibody-epr18982-13-immunoprecipitation.jpg)
Immunoprecipitation - Anti-n-Myc/MYCN antibody [EPR18982-13] (ab189528)n-Myc/MYCN was immunoprecipitated from 2μg of HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysates with ab189528 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab16898 at 1/1000 dilution.
Goat Anti-Mouse IgG (H+L) Peroxidase Conjugate, was used as secondary antibody at 1/10000 dilution.
Lane 1: HEK-293 whole cell lysate transfected with GFP-tagged n-Myc/MYCN construct, 10ug (Input).
Lane 2: ab189528 IP in HEK-293 whole cell lysate transfected with GFP-tagged n-Myc/MYCN construct.
Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab189528 IP in HEK-293 whole cell lysate transfected with GFP-tagged n-Myc/MYCN construct.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
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![Immunoprecipitation - Anti-n-Myc/MYCN antibody [EPR18982-13] (ab189528)](https://www.abcam.com/ps/products/189/ab189528/Images/ab189528-269315-anti-n-myc-antibody-epr18982-13-immunoprecipitation.jpg)
Immunoprecipitation - Anti-n-Myc/MYCN antibody [EPR18982-13] (ab189528)n-Myc/MYCN was immunoprecipitated from 2μg of HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysates with ab189528 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab290 at 1/2000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: HEK-293 whole cell lysate transfected with n-Myc/MYCN, 10ug (Input).
Lane 2: ab189528 IP in HEK-293 whole cell lysate transfected with GFP-tagged n-Myc/MYCN construct.
Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab189528 IP in HEK-293 whole cell lysate transfected with GFP-tagged n-Myc/MYCN construct.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
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![Anti-n-Myc/MYCN antibody [EPR18982-13] (ab189528)](https://www.abcam.com/ps/products/189/ab189528/Images/ab189528-5-benefits-of-recombinant-antibodies.png)
Anti-n-Myc/MYCN antibody [EPR18982-13] (ab189528)
