Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Tau antibody [EPR22524-95] (ab254256)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22524-95] to Tau
  • Suitable for: IP, IHC-Fr, WB, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Tau antibody [EPR22524-95]
    See all Tau primary antibodies

  • Description

    Rabbit monoclonal [EPR22524-95] to Tau

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    IHC-P
    Mouse
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human and mouse brain lysate. IHC-P: Mouse cerebral cortex and hippocampus tissue. Human cerebral cortex tissue. IHC-Fr: Mouse cerebral cortex tissue. IP: Human brain lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Tau antibody [EPR22524-95] (ab254256)
    Western blot - Anti-Tau antibody [EPR22524-95] (ab254256)
    All lanes : Anti-Tau antibody [EPR22524-95] (ab254256) at 1/1000 dilution

    Lane 1 : Human brain lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate
    Lane 4 : Human spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 76 kDa
    Observed band size: 50-70 kDa
    why is the actual band size different from the predicted?


    Exposure time: 8 seconds


    Negative control: human fetal heart, human fetal kidney, human spleen. (PMID: 24386422).

    The molecular weight observed is consistent with what has been described in the literature (PMID: 24386422).

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Tau antibody [EPR22524-95] (ab254256)
    Western blot - Anti-Tau antibody [EPR22524-95] (ab254256)
    All lanes : Anti-Tau antibody [EPR22524-95] (ab254256) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Mouse spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 76 kDa
    Observed band size: 50-70 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Negative control: mouse heart, mouse kidney, mouse spleen. (PMID: 24386422).

    The molecular weight observed is consistent with what has been described in the literature (PMID: 24386422).

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-Tau antibody [EPR22524-95] (ab254256)
    Immunoprecipitation - Anti-Tau antibody [EPR22524-95] (ab254256)

    Tau was immunoprecipitated from 0.35 mg human brain lysate with ab254256 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab254256 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.

    Lane 1: Human brain lysate 10 µg (Input).
    Lane 2: ab254256 IP in human brain lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254256 in human brain lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 10 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] (ab254256)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] (ab254256)

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling Tau with ab254256 at 1/4000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse hippocampus (PMID: 22961084) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab254256 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Frozen sections) - Anti-Tau antibody [EPR22524-95] (ab254256)
    Immunohistochemistry (Frozen sections) - Anti-Tau antibody [EPR22524-95] (ab254256)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebral cortex tissue labeling Tau with ab254256 at 1/100 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. Positive staining on mouse cerebral cortex (PMID: 22961084) is observed. Counterstained with DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1,000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] (ab254256)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] (ab254256)

    Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling Tau with ab254256 at 1/4000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebral cortex (PMID: 22961084) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab254256 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] (ab254256)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] (ab254256)

    Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling Tau with ab254256 at 1/4000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebral cortex (PMID: 22961084) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab254256 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Anti-Tau antibody [EPR22524-95] (ab254256)
    Anti-Tau antibody [EPR22524-95] (ab254256)

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