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Signal Transduction Metabolism

Anti-TUB 1 antibody [EPR8241] - BSA and Azide free (ab248353)

Anti-TUB 1 antibody [EPR8241] - BSA and Azide free (ab248353)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8241] to TUB 1 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-TUB 1 antibody [EPR8241] - BSA and Azide free
    See all TUB 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR8241] to TUB 1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248353 is the carrier-free version of ab131034.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Dissociation constant (KD)

    KD = 5.43 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8241
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Metabolism
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

Images

  • Western blot - Anti-TUB 1 antibody [EPR8241] - BSA and Azide free (ab248353)
    Western blot - Anti-TUB 1 antibody [EPR8241] - BSA and Azide free (ab248353)
    All lanes : Anti-TUB 1 antibody [EPR8241] (ab131034) at 1/1000 dilution

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : Saos-2 cell lysate
    Lane 3 : C6 cell lysate
    Lane 4 : L6 cell lysate
    Lane 5 : Neuro-2a cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 56 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab131034, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUB 1 antibody [EPR8241] - BSA and Azide free (ab248353)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUB 1 antibody [EPR8241] - BSA and Azide free (ab248353)
    This data was developed using ab131034, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labelling TUB 1 with ab131034 antibody at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • OI-RD Scanning - Anti-TUB 1 antibody [EPR8241] - BSA and Azide free (ab248353)
    OI-RD Scanning - Anti-TUB 1 antibody [EPR8241] - BSA and Azide free (ab248353)
    This data was developed using ab131034, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-TUB 1 antibody [EPR8241] - BSA and Azide free (ab248353)
    Anti-TUB 1 antibody [EPR8241] - BSA and Azide free (ab248353)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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