Anti-FADS2 antibody (ab232898)
Key features and details
- Rabbit polyclonal to FADS2
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-FADS2 antibody
See all FADS2 primary antibodies -
Description
Rabbit polyclonal to FADS2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human, Pig
Predicted to work with: Cynomolgus monkey
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Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human FADS2 aa 1-150. Expressed in E.coli.
Database link: O95864 -
Positive control
- WB: Recombinant human FADS2 protein; HepG2 cell lysate; Pig liver and large intestine lysates; Rat pancreas lysate. IHC-P: Human breast cancer, cerebrum, liver, glioma, lung cancer and kidney tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FADS2 antibody (ab232898)All lanes : Anti-FADS2 antibody (ab232898) at 1 µg/ml
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 2 : Pig liver lysate
Lane 3 : Pig large intestine lysate
Lane 4 : Rat pancreas lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 52 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody (ab232898)Formalin-fixed, paraffin-embedded human breast cancer tissue stained for FADS2 using ab232898 at 30 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody (ab232898)Formalin-fixed, paraffin-embedded human cerebrum tissue stained for FADS2 using ab232898 at 30 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody (ab232898)Formalin-fixed, paraffin-embedded human liver tissue stained for FADS2 using ab232898 at 30 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody (ab232898)Formalin-fixed, paraffin-embedded human glioma tissue stained for FADS2 using ab232898 at 30 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody (ab232898)Formalin-fixed, paraffin-embedded human lung cancer tissue stained for FADS2 using ab232898 at 30 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody (ab232898)Formalin-fixed, paraffin-embedded human kidney tissue stained for FADS2 using ab232898 at 30 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Western blot - Anti-FADS2 antibody (ab232898)Anti-FADS2 antibody (ab232898) at 1 µg/ml + Recombinant human FADS2 protein
Secondary
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?19kDa is oberserved because this is image is produced with part of the FADS2 protein (aa 1-130) and not from full length.
