Anti-FADS2 antibody (ab72189)
Key features and details
- Rabbit polyclonal to FADS2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FADS2 antibody
See all FADS2 primary antibodies -
Description
Rabbit polyclonal to FADS2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Monkey, Baboon, Orangutan
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in the following lysates: Human liver tissue lysate; Human brain tissue lysates; HeLa whole cell lysate; Hep G2 whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab72189 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 52 kDa). IP Use at an assay dependent concentration. Target
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Function
Component of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3). Catalyzes the first and rate limiting step in this pathway which is the desaturation of LA (18:2n-6) and ALA (18:3n-3) into gamma-linoleic acid (GLA) (18:3n-6) and stearidonic acid (18:4n-3) respectively and other desaturation steps. Highly unsaturated fatty acids (HUFA) play pivotal roles in many biological functions. It catalizes as well the introduction of a cis double bond in palmitate to produce the mono-unsaturated fatty acid sapienate, the most abundant fatty acid in sebum. -
Tissue specificity
Expressed in a wide array of tissues, highest expression is found in liver followed by brain, lung, heart, and retina. A lower level is found in breast tumor when compared with normal tissues; lowest levels were found in patients with poor prognostic index. -
Pathway
Lipid metabolism; polyunsaturated fatty acid biosynthesis. -
Sequence similarities
Belongs to the fatty acid desaturase family.
Contains 1 cytochrome b5 heme-binding domain. -
Developmental stage
Found in fetal heart. -
Domain
The histidine box domains may contain the active site and/or be involved in metal ion binding. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 9415 Human
- Omim: 606149 Human
- SwissProt: O95864 Human
- SwissProt: Q5REA7 Orangutan
- Unigene: 502745 Human
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Alternative names
- D6D antibody
- Delta(6) desaturase antibody
- Delta(6) fatty acid desaturase antibody
see all
Images
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Immunoprecipitation - Anti-FADS2 antibody (ab72189)FADS2 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Rabbit polyclonal to FADS2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab72189.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 45kDa:FADS2. -
Western blot - Anti-FADS2 antibody (ab72189)All lanes : Anti-FADS2 antibody (ab72189) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : Human brain tissue lysate - total protein (ab29466)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 62.5 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
ab72189 detects a strong band at 45 kDa in human liver and brain tissue lysates, aswell as hela and hepG2 whole cell lysates. This antibody was raised against an immunogen that is predicted to cross react with all known isoforms of Fatty acid desaturase 2 (FADS2). FADS2 has three isoforms, isoform one has a molecular weight of 52 kDa with isoforms two and three a molecular weight of 49 and 45 respectively(SwissProt data). The band we have observed is thought to represent isoform 3 of FADS2 (45 kDa).
Protocols
Datasheets and documents
References (2)
ab72189 has been referenced in 2 publications.
- Tsachaki M et al. Impact of 17ß-HSD12, the 3-ketoacyl-CoA reductase of long-chain fatty acid synthesis, on breast cancer cell proliferation and migration. Cell Mol Life Sci N/A:N/A (2019). PubMed: 31302749
- Naudí A et al. Region-specific vulnerability to lipid peroxidation and evidence of neuronal mechanisms for polyunsaturated fatty acid biosynthesis in the healthy adult human central nervous system. Biochim Biophys Acta 1862:485-495 (2017). WB ; Human . PubMed: 28185952
Images
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Immunoprecipitation - Anti-FADS2 antibody (ab72189)FADS2 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Rabbit polyclonal to FADS2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab72189.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 45kDa:FADS2. -
Western blot - Anti-FADS2 antibody (ab72189)All lanes : Anti-FADS2 antibody (ab72189) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : Human brain tissue lysate - total protein (ab29466)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 62.5 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
ab72189 detects a strong band at 45 kDa in human liver and brain tissue lysates, aswell as hela and hepG2 whole cell lysates. This antibody was raised against an immunogen that is predicted to cross react with all known isoforms of Fatty acid desaturase 2 (FADS2). FADS2 has three isoforms, isoform one has a molecular weight of 52 kDa with isoforms two and three a molecular weight of 49 and 45 respectively(SwissProt data). The band we have observed is thought to represent isoform 3 of FADS2 (45 kDa).
