Anti-TUB 1 antibody [EPR8241] (ab131034)
![Anti-TUB 1 antibody [EPR8241] (ab131034)](https://www.abcam.com/ps/products/131/ab131034/Images/TUB-1-Primary-antibodies-ab131034-1.jpg "Anti-TUB 1 antibody [EPR8241] (ab131034)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8241] to TUB 1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-TUB 1 antibody [EPR8241]
See all TUB 1 primary antibodies -
Description
Rabbit monoclonal [EPR8241] to TUB 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to residues near the N-terminus of Human TUB 1 (UniProt P50607).
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Positive control
- SH-SY5Y, Saos-2, C6, L6, and Neuro-2a cell lysates; Human astrocytoma tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 5.43 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8241 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TUB 1 antibody [EPR8241] (ab131034)All lanes : Anti-TUB 1 antibody [EPR8241] (ab131034) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : Saos-2 cell lysate
Lane 3 : C6 cell lysate
Lane 4 : L6 cell lysate
Lane 5 : Neuro-2a cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 56 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUB 1 antibody [EPR8241] (ab131034)](https://www.abcam.com/ps/products/131/ab131034/Images/TUB-1-Primary-antibodies-ab131034-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUB 1 antibody [EPR8241] (ab131034)Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labelling TUB 1 with ab131034 antibody at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-TUB 1 antibody [EPR8241] (ab131034)](https://www.abcam.com/ps/products/131/ab131034/Images/TUB-1-Primary-antibodies-ab131034-3.jpg)
OI-RD Scanning - Anti-TUB 1 antibody [EPR8241] (ab131034) -
![Anti-TUB 1 antibody [EPR8241] (ab131034)](https://www.abcam.com/ps/products/131/ab131034/Images/ab131034-4-benefits-of-recombinant-antibodies.png)
Anti-TUB 1 antibody [EPR8241] (ab131034)