Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ACSM2A antibody [EPR13330] - BSA and Azide free (ab250424)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13330] to ACSM2A - BSA and Azide free
  • Suitable for: IHC-P, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-ACSM2A antibody [EPR13330] - BSA and Azide free
    See all ACSM2A primary antibodies

  • Description

    Rabbit monoclonal [EPR13330] to ACSM2A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IP, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250424 is the carrier-free version of ab181204.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ACSM2A antibody [EPR13330] - BSA and Azide free (ab250424)
    Western blot - Anti-ACSM2A antibody [EPR13330] - BSA and Azide free (ab250424)
    All lanes : Anti-ACSM2A antibody [EPR13330] (ab181204) at 1/2000 dilution

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : Human fetal kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 64 kDa



    This data was developed using ab181204, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    59 KDa: Mature form

    64 KDa: Full length.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSM2A antibody [EPR13330] - BSA and Azide free (ab250424)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSM2A antibody [EPR13330] - BSA and Azide free (ab250424)
    This data was developed using ab181204, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling ACSM2A with ab181204 at a 1/250 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSM2A antibody [EPR13330] - BSA and Azide free (ab250424)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSM2A antibody [EPR13330] - BSA and Azide free (ab250424)
    This data was developed using ab181204, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ACSM2A with ab181204 at a 1/250 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-ACSM2A antibody [EPR13330] - BSA and Azide free (ab250424)
    Immunoprecipitation - Anti-ACSM2A antibody [EPR13330] - BSA and Azide free (ab250424)
    This data was developed using ab181204, the same antibody clone in a different buffer formulation.Immunoprecipitation of Human fetal kidney labeling ACSM2A using ab181204 at a 1/50 dilution (lane 1). Lane 2 shows the negative control. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as a secondary antibody at a dilution of 1/1500. Blocking/dilution buffer and concentration: 5% NFDM/TBST.59 KDa: Mature form64 KDa: Full length
  • Anti-ACSM2A antibody [EPR13330] - BSA and Azide free (ab250424)
    Anti-ACSM2A antibody [EPR13330] - BSA and Azide free (ab250424)

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