Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free (ab250676)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17680] to AKT1 (phospho S124) - BSA and Azide free
- Suitable for: ELISA, Dot blot, IP, WB
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-AKT1 (phospho S124) antibody [EPR17680] - BSA and Azide free
See all AKT1 primary antibodies -
Description
Rabbit monoclonal [EPR17680] to AKT1 (phospho S124) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, Dot blot, IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
Ab250676 is the carrier-free version of ab183556. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250676 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17680 -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556) at 1/1000 dilution
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate, untreated
Lane 2 : MCF7 whole cell lysate, treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 15 secondsThis data was developed using ab183556, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
All lanes : Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556) at 1/10000 dilution
Lane 1 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate, untreated
Lane 2 : NIH/3T3 whole cell lysate, treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 3 minutesThis data was developed using ab183556, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
All lanes : Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556) at 1/10000 dilution
Lane 1 : C6 (Rat glial tumor cells) whole cell lysate, untreated
Lane 2 : C6 whole cell lysate, treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 3 minutesThis data was developed using ab183556, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
All lanes : Anti-AKT1 (phospho S124) antibody [EPR17680] (ab183556) at 1/1000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : Human fetal kidney tissue lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : Rat heart tissue lysate
Lane 6 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 3 minutesThis data was developed using ab183556, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
This data was developed using ab183556, the same antibody clone in a different buffer formulation.
Immunoprecipitation of AKT1 from 1mg of MCF7 (Human breast adenocarcinoma cell line) whole cell lysate achieved using ab183556 at 1/100 dilution.
Lane 1: Input: 10µg of MCF7 whole cell lysate.
Lane 2: MCF7 whole cell lysate following IP with ab183556.
Lane 3: negative control: IP using Rabbit monoclonal IgG (ab172730) instead of ab183556 in MCF7 whole cell lysate.
Western blot was performed using ab183556 at 1/1000 dilution.
An Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.
-
This data was developed using ab183556, the same antibody clone in a different buffer formulation.Dot blot analysis of AKT1 (phospho S124) labeled with ab183556 at 1/1000 dilution.
Lane 1: phospho peptide
Lane 2: non phospho peptide
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 was used as secondary antibody.
Exposure time: 3 minutes.Blocking and Diluting buffer buffer and concentration: 5% NFDM/TBST -
This data was developed using ab183556, the same antibody clone in a different buffer formulation.ELISA image showing specificity of ab183556 to AKT1 (phospho S124) peptide only.Peptides concentration: 1000 ng/ml.ab183556 working dilution: 1/1700.Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500.
-