Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Tuberin (phospho S1254) antibody [EPR5682] - BSA and Azide free (ab248503)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5682] to Tuberin (phospho S1254) - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Tuberin (phospho S1254) antibody [EPR5682] - BSA and Azide free
    See all Tuberin primary antibodies

  • Description

    Rabbit monoclonal [EPR5682] to Tuberin (phospho S1254) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody detects Tuberin only when phosphorylated at Serine 1254.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248503 is the carrier-free version of ab133454 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248503 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Tuberin (phospho S1254) antibody [EPR5682] - BSA and Azide free (ab248503)
    Western blot - Anti-Tuberin (phospho S1254) antibody [EPR5682] - BSA and Azide free (ab248503)
    All lanes : Anti-Tuberin (phospho S1254) antibody [EPR5682] (ab133454) at 1/10000 dilution

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : SH-SY5Y cell lysate treated with Okadaic Acid and Calyculin A

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 201 kDa
    Observed band size: 180 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab133454, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tuberin (phospho S1254) antibody [EPR5682] - BSA and Azide free (ab248503)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tuberin (phospho S1254) antibody [EPR5682] - BSA and Azide free (ab248503)
    This data was developed using ab133454, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling Tuberin Phospho S1254 with ab133454 at 1/50 dilution Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • OI-RD Scanning - Anti-Tuberin (phospho S1254) antibody [EPR5682] - BSA and Azide free (ab248503)
    OI-RD Scanning - Anti-Tuberin (phospho S1254) antibody [EPR5682] - BSA and Azide free (ab248503)
    This data was developed using ab133454, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Tuberin (phospho S1254) antibody [EPR5682] - BSA and Azide free (ab248503)
    Anti-Tuberin (phospho S1254) antibody [EPR5682] - BSA and Azide free (ab248503)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.