Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] - BSA and Azide free (ab256225)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2259Y] to EGFR (phospho S1046 + S1047) - BSA and Azide free
  • Suitable for: WB, IP, ICC/IF, Dot blot
  • Reacts with: Human

Overview

  • Product name

    Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] - BSA and Azide free
    See all EGFR primary antibodies

  • Description

    Rabbit monoclonal [EP2259Y] to EGFR (phospho S1046 + S1047) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, ICC/IF, Dot blotmore details
    Unsuitable for: Flow Cyt or IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: A431 cells treated with EGF. IP: A431 treated with EGF whole cell lysate. Dot Blot: EGFR (pS1046/pS1047) phospho peptide.

  • General notes

    ab256225 is the carrier-free version of ab76300 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab256225 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] - BSA and Azide free (ab256225)
    Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] - BSA and Azide free (ab256225)

    Immunocytochemistry/Immunofluorescence analysis of untreated and EGF (100ng/mL for 10 minutes) treated A431 cells labelling EGFR (phospho S1046 + S1047) with ab76300 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. The cells were co-stained with ab195889, an Alexa Fluor® 594-conjugated mouse anti-tubulin (1/200). Nuclei counterstained with DAPI (blue).

    Control 1: untreated A431 cells incubated with PBS instead of primary antibody followed by incubation with ab150077 Alexa Fluor® 488 goat anti-rabbit IgG.
    Control 2: EGF treated A431 cells incubated with PBS instead of primary antibody followed by incubation with ab150077 Alexa Fluor® 488 goat anti-rabbit IgG.

    This data was developed using the same antibody clone in a different buffer formulation containing Tris Glycine, BSA, glycerol, and sodium azide (ab76300).

  • Immunoprecipitation - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] - BSA and Azide free (ab256225)
    Immunoprecipitation - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] - BSA and Azide free (ab256225)

    This data was developed using ab76300, the same antibody clone in a different buffer formulation.
    Purified ab76300 at 1/40 dilution (2µg) immunoprecipitating EGFR in A431 treated with EGF whole cell lysate.
    Lane 1 (input): A431 (Human epidermoid carcinoma epithelial cell) treated with EGF whole cell lysate 10µg
    Lane 2 (+): ab76300 + A431 treated with EGF whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab76300 in A431 treated with EGF whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 180 kDa

  • Dot Blot - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] - BSA and Azide free (ab256225)
    Dot Blot - Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] - BSA and Azide free (ab256225)

    This data was developed using the same antibody clone in a different buffer formulation (ab76300).

    Primary antibody: ab76300 at a dilution of 1/1000.

    Secondary antibody: Peroxidase conjugated-goat anti-rabbit IgG, (H+L) at a dilution of 1/2500.

    Blocking and dilution buffer: 5% NFDM/TBST

    Lane 1: EGFR (pS1046/pS1047) phospho peptide.
    Lane 2: EGFR non-phospho peptide.

    Exposure time: 3 minutes.

  • Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] - BSA and Azide free (ab256225)
    Anti-EGFR (phospho S1046 + S1047) antibody [EP2259Y] - BSA and Azide free (ab256225)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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