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Anti-IL-1RAcP antibody [EPR22678-67] - BSA and Azide free (ab256764)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22678-67] to IL-1RAcP - BSA and Azide free
  • Suitable for: ELISA, WB, Flow Cyt, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-IL-1RAcP antibody [EPR22678-67] - BSA and Azide free
    See all IL-1RAcP primary antibodies

  • Description

    Rabbit monoclonal [EPR22678-67] to IL-1RAcP - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ELISA, WB, Flow Cyt, IPmore details
    Unsuitable for: ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: HeLa and HepG2 cells. IP: KARPAS-299 whole cell lysate.

  • General notes

    ab256764 is the carrier-free version of ab256461 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab256764 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-IL-1RAcP antibody [EPR22678-67] - BSA and Azide free (ab256764)
    Flow Cytometry - Anti-IL-1RAcP antibody [EPR22678-67] - BSA and Azide free (ab256764)

    This data was developed using the same antibody clone in a different buffer formulation (ab256461)

    Flow cytometry overlay histogram showing wild-type HeLa (green line) and IL1RAP knockout HeLa cells (ab273375) stained with ab256461 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab256461) (1x106 in 100μl at 10 μ/ml) for 30 min at 4°C.

    The secondary antibody Goat anti-rabbit IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150081) was used at 1/2000 for 30 min at 4°C.

    Isotype control antibody was Rabbit IgG (monoclonal) (ab172730) used at the same concentration and conditions as the primary antibody (wild-type HeLa - black line; IL1RAP knockout HeLa - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

    Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

  • Immunoprecipitation - Anti-IL-1RAcP antibody [EPR22678-67] - BSA and Azide free (ab256764)
    Immunoprecipitation - Anti-IL-1RAcP antibody [EPR22678-67] - BSA and Azide free (ab256764)

    IL-1RAcP was immunoprecipitated from 0.35 mg KARPAS-299 (human anaplastic large cell lymphoma) whole cell lysate 10μg with ab256461 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256461 at a 1/1000 dilution (0.59 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.


    Lane 1: KARPAS-299 (human anaplastic large cell lymphoma) whole cell lysate 10μg.


    Lane 2: ab256461 IP in KARPAS-299 whole cell lysate.


    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256461 in KARPAS-299 whole cell lysate.


    Blocking/Dilution buffer: 5% NFDM/TBST.


    Exposure time: 75 secs.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256461).
  • Flow Cytometry - Anti-IL-1RAcP antibody [EPR22678-67] - BSA and Azide free (ab256764)
    Flow Cytometry - Anti-IL-1RAcP antibody [EPR22678-67] - BSA and Azide free (ab256764)

    Flow cytometric analysis of Raji (Human burkitt's lymphoma b lymphocyte, Left panel) and HepG2 (Human hepatocellular carcinoma epithelial cell, Right panel) cells labeling IL-1RAcP with ab256461 at a 1/60 dilution (1μg, red), compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at a 1/2000 dilution was used as the secondary antibody. Negative control: Raji (PMID: 30514753). Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256461).
  • ELISA - Anti-IL-1RAcP antibody [EPR22678-67] - BSA and Azide free (ab256764)
    ELISA - Anti-IL-1RAcP antibody [EPR22678-67] - BSA and Azide free (ab256764)

    This data was developed using ab256461, the same antibody clone in a different buffer formulation.

    ELISA analysis of Hu IL1RAP recombinant protein at 1000 ng/mL with ab256461. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
  • Anti-IL-1RAcP antibody [EPR22678-67] - BSA and Azide free (ab256764)
    Anti-IL-1RAcP antibody [EPR22678-67] - BSA and Azide free (ab256764)

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