Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD32B antibody [EP888Y] (ab45143)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP888Y] to CD32B
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-CD32B antibody [EP888Y]
    See all CD32B primary antibodies

  • Description

    Rabbit monoclonal [EP888Y] to CD32B

  • Host species

    Rabbit

  • Specificity

    This antibody has weak cross-reactivity with CD32A and CD32C. It detects weak signal in CD32A and CD32C over-expressed lysates at the dilution of 1/200 under the exposure time of 3 minutes, but detects strong band in CD32B over-expressed lysates at the dilution of 1:2000 under the exposure time of 15 seconds.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human CD32B aa 250-350 (C terminal).

  • Positive control

    • Daudi cell lysate, human lymph node tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B antibody [EP888Y] (ab45143)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B antibody [EP888Y] (ab45143)

    Unpurified ab45143 staining human CD32 (1/100) in human lymph node by immunohistochemistry using paraffin embedded tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-CD32B antibody [EP888Y] (ab45143)
    Western blot - Anti-CD32B antibody [EP888Y] (ab45143)
    All lanes : Anti-CD32B antibody [EP888Y] (ab45143) at 1/10000 dilution (purified)

    Lane 1 : Human thyroid lysate
    Lane 2 : Daudi whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Observed band size: 40-44 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B antibody [EP888Y] (ab45143)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32B antibody [EP888Y] (ab45143)
    Immunohistochemical staining of paraffin embedded human spleen with purified ab45143 at a working dilution of 1/1000. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Western blot - Anti-CD32B antibody [EP888Y] (ab45143)
    Western blot - Anti-CD32B antibody [EP888Y] (ab45143)
    Lanes 1-2 & 5-6 : Anti-CD32B antibody [EP888Y] (ab45143) at 1/200 dilution (unpurified)
    Lanes 3-4 : Anti-CD32B antibody [EP888Y] (ab45143) at 1/2000 dilution (unpurified)

    Lane 1 : Human CD32A overexpressed lysate
    Lanes 2 & 4 & 6 : Non-transfected lysate
    Lane 3 : Human CD32B overexpressed lysate
    Lane 5 : Human CD32C overexpressed lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Observed band size: 39 kDa why is the actual band size different from the predicted?



    Exposure times: Lanes 1 and 2: 3 minutes, Lanes 3 and 4: 15 seconds, Lanes 5 and 6: 3 minutes.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-CD32B antibody [EP888Y] (ab45143)
    Western blot - Anti-CD32B antibody [EP888Y] (ab45143)
    Anti-CD32B antibody [EP888Y] (ab45143) at 1/50000 dilution (unpurified) + Daudi cell lysate at 10 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Observed band size: 49-55 kDa why is the actual band size different from the predicted?

  • Anti-CD32B antibody [EP888Y] (ab45143)
    Anti-CD32B antibody [EP888Y] (ab45143)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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