Human alpha Fetoprotein ELISA Kit (ab108631)
Key features and details
- Sensitivity: 2 ng/ml
- Range: 2 ng/ml - 300 ng/ml
- Sample type: Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human alpha Fetoprotein ELISA Kit
See all alpha 1 Fetoprotein kits -
Detection method
Colorimetric -
Sample type
Serum -
Assay type
Sandwich (quantitative) -
Sensitivity
= 2 ng/ml -
Range
2 ng/ml - 300 ng/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s alpha Fetoprotein (AFP) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of alpha Fetoprotein in Human serum.
A 96-well plate has been precoated with anti-alpha Fetoprotein antibodies. Samples and standards and are added to the wells, where alpha Fetoprotein in the sample and standards binds to the precoated antibody. After incubation and washing, added Anti-alpha Fetoprotein-HRP conjugate binds to the antibody-alpha Fetoprotein complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of alpha Fetoprotein in the sample and the intensity is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests alpha Fetoprotein Standard - 0 ng/mL 1 x 0.5ml alpha Fetoprotein Standard - 150 ng/mL 1 x 0.5ml alpha Fetoprotein Standard - 20 ng/mL 1 x 0.5ml alpha Fetoprotein Standard - 300 ng/mL 1 x 0.5ml alpha Fetoprotein Standard - 5 ng/mL 1 x 0.5ml alpha Fetoprotein Standard - 50 ng/mL 1 x 0.5ml Anti-alpha Fetoprotein Coated Microplate (12 x 8 wells) 1 unit Anti-alpha Fetoprotein HRP Conjugate 1 x 18ml Stop Solution 1 x 11ml TMB Reagent 1 x 11ml Zero Buffer 1 x 13ml -
Research areas
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Function
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. -
Tissue specificity
Plasma. Synthesized by the fetal liver and yolk sac. -
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Developmental stage
Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma. -
Post-translational
modificationsIndependent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Afp
- AFPD
- Alpha fetoglobulin
see all -
Database links
- Entrez Gene: 174 Human
- Omim: 104150 Human
- SwissProt: P02771 Human
- Unigene: 518808 Human
Images
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Typical Standard Curve
Representative standard curve using ab108631
