Anti-CD32B (phospho Y292) antibody (ab74092)
Key features and details
- Rabbit polyclonal to CD32B (phospho Y292)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CD32B (phospho Y292) antibody
See all CD32B primary antibodies -
Description
Rabbit polyclonal to CD32B (phospho Y292) -
Host species
Rabbit -
Specificity
ab74092 detects endogenous levels of CD32 only when phosphorylated at tyrosine 292. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic phosphopeptide derived from human CD32B around the phosphorylation site of tyrosine 292 (I-T-YP-S-L). (SwissProt P31994).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab74092 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CD32B (phospho Y292) antibody (ab74092)All lanes : Anti-CD32B (phospho Y292) antibody (ab74092) at 1/500 dilution
Lane 1 : K562 cell extracts treated
with PMA (125ng/ml, 30mins)
Lane 2 : K562 cell extracts treated
with PMA (125ng/ml, 30mins) with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 34 kDa
Observed band size: 34 kDa
