Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Myeloperoxidase antibody [EPR4792] - BSA and Azide free (ab247766)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4792] to Myeloperoxidase - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Myeloperoxidase antibody [EPR4792] - BSA and Azide free
    See all Myeloperoxidase primary antibodies

  • Description

    Rabbit monoclonal [EPR4792] to Myeloperoxidase - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody recognizes Myeloperoxidase (85-90 kDa) and its light chain (15 kDa).

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247766 is the carrier-free version of ab109116 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247766 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Myeloperoxidase antibody [EPR4792] - BSA and Azide free (ab247766)
    Western blot - Anti-Myeloperoxidase antibody [EPR4792] - BSA and Azide free (ab247766)
    Anti-Myeloperoxidase antibody [EPR4792] (ab109116) at 1/1000 dilution + HL-60 cell lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 84 kDa
    Observed band size: 85-90 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 15 kDa (possible cleavage fragment)



    This data was developed using ab109116, the same antibody clone in a different buffer formulation.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] - BSA and Azide free (ab247766)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] - BSA and Azide free (ab247766)

    This data was developed using ab109116, the same antibody clone in a different buffer formulation.

    ab109116, at a 1/250 dilution, staining Myeloperoxidase in paraffin embedded Human colon tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] - BSA and Azide free (ab247766)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] - BSA and Azide free (ab247766)
    This data was developed using ab109116, the same antibody clone in a different buffer formulation.ab109116, at a 1/250 dilution, staining Myeloperoxidase in paraffin embedded Human lung tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] - BSA and Azide free (ab247766)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] - BSA and Azide free (ab247766)
    This data was developed using ab109116, the same antibody clone in a different buffer formulation.ab109116, at a 1/250 dilution, staining Myeloperoxidase in paraffin embedded Human spleen tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] - BSA and Azide free (ab247766)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] - BSA and Azide free (ab247766)
    This data was developed using ab109116, the same antibody clone in a different buffer formulation.ab109116 showing positive staining in Normal liver tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] - BSA and Azide free (ab247766)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] - BSA and Azide free (ab247766)
    This data was developed using ab109116, the same antibody clone in a different buffer formulation.ab109116 showing positive staining in Bone marrow tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] - BSA and Azide free (ab247766)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] - BSA and Azide free (ab247766)
    This data was developed using ab109116, the same antibody clone in a different buffer formulation.ab109116 showing negative staining in Skeletal muscle tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-Myeloperoxidase antibody [EPR4792] - BSA and Azide free (ab247766)
    Anti-Myeloperoxidase antibody [EPR4792] - BSA and Azide free (ab247766)

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