Anti-FLAP antibody (ab53536)
Key features and details
- Goat polyclonal to FLAP
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FLAP antibody
See all FLAP primary antibodies -
Description
Goat polyclonal to FLAP -
Host species
Goat -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Horse, Cynomolgus monkey, Rhesus monkey
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Immunogen
Synthetic peptide:
C-HKVEHESRTQNGR
, corresponding to internal sequence amino acids 28-40 of Human FLAP -
Positive control
- Recombinant Human FLAP protein (ab114639) can be used as a positive control in WB. U937 or Peripheral Blood Mononucleocyte lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FLAP antibody (ab53536)Anti-FLAP antibody (ab53536) at 0.1 µg/ml + Human U937 cell lysate (35µg protein in RIPA buffer).
Predicted band size: 18 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLAP antibody (ab53536)ab53536 (3.75µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLAP antibody (ab53536)ab53536 (3.75µg/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. -
Immunocytochemistry/ Immunofluorescence - Anti-FLAP antibody (ab53536)ICC/IF image of ab53536 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53536, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLAP antibody (ab53536)
