Anti-PGK1 antibody (ab38007)
Key features and details
- Rabbit polyclonal to PGK1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PGK1 antibody
See all PGK1 primary antibodies -
Description
Rabbit polyclonal to PGK1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Saccharomyces cerevisiae
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Immunogen
Synthetic peptide conjugated to KLH selected within aa 100~200 of human PGK1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
This antibody is purified through a protein G column, eluted out with both high and low pH buffers, neutralized immediately after elution then followed by dialysis against PBS. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PGK1 antibody (ab38007)All lanes : Anti-PGK1 antibody (ab38007) at 1/100 dilution
Lane 1 : Mouse stomach tissue lysate
Lane 2 : HepG2 cell lysate
Predicted band size: 44 kDa
Observed band size: 44 kDa
Additional bands at: 60 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGK1 antibody (ab38007)ab38007 at 1/100 dilution staining PGK1 in human Hepatocarcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.
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Immunocytochemistry/ Immunofluorescence - Anti-PGK1 antibody (ab38007)ICC/IF image of ab38007 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38007, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
