Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Apo-D antibody [EPR22967-72] (ab256496)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22967-72] to Apo-D
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Apo-D antibody [EPR22967-72]
    See all Apo-D primary antibodies

  • Description

    Rabbit monoclonal [EPR22967-72] to Apo-D

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human plasma, Human heart and Human kidney lysates. IHC-P: Human colon and Human breast cancer tissues. IP: Human plasma and Human placenta cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Apo-D antibody [EPR22967-72] (ab256496)
    Western blot - Anti-Apo-D antibody [EPR22967-72] (ab256496)
    All lanes : Anti-Apo-D antibody [EPR22967-72] (ab256496) at 1/1000 dilution

    Lane 1 : Human plasma
    Lane 2 : Human heart tissue lysate
    Lane 3 : Human kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 21 kDa
    Observed band size: 22-35 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The smeared bands between 22-35 kDa are mainly caused by different levels of glycosylation (PMID: 26829325). 

    Exposure time: Lane 1: 26 seconds; Lanes 2-3: 3 minutes.

  • Immunoprecipitation - Anti-Apo-D antibody [EPR22967-72] (ab256496)
    Immunoprecipitation - Anti-Apo-D antibody [EPR22967-72] (ab256496)

    Apo-D was immunoprecipitated from 0.35 mg Human placenta lysate with ab256496 at 1/40 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256496 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: Human placenta lysate 10ug

    Lane 2: ab256496 IP in Human placenta lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256496 in Human placenta lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Immunoprecipitation - Anti-Apo-D antibody [EPR22967-72] (ab256496)
    Immunoprecipitation - Anti-Apo-D antibody [EPR22967-72] (ab256496)

    Apo-D was immunoprecipitated from 0.35 mg Human plasma lysate with ab256496 at 1/40 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256496 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: Human plasma lysate 10ug

    Lane 2: ab256496 IP in Human plasma lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256496 in Human plasma lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR22967-72] (ab256496)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR22967-72] (ab256496)

    Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling Apo-D with ab256496 at 1/2000 dilution (0.35ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human breast cancer (PMID:18330697, 18505051). Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR22967-72] (ab256496)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR22967-72] (ab256496)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Apo-D with ab256496 at 1/2000 dilution (0.35ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in stromal cells of human colon (PMID: 23296401). Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Anti-Apo-D antibody [EPR22967-72] (ab256496)
    Anti-Apo-D antibody [EPR22967-72] (ab256496)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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