Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Apo-D antibody [EPR22967-72] (ab256496)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22967-72] to Apo-D
Suitable for: WB, IHC-P, IP
Reacts with: Human

Product name

Anti-Apo-D antibody [EPR22967-72]
See all Apo-D primary antibodies
Description

Rabbit monoclonal [EPR22967-72] to Apo-D
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human plasma, Human heart and Human kidney lysates. IHC-P: Human colon and Human breast cancer tissues. IP: Human plasma and Human placenta cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22967-72
Isotype

IgG
Research areas

Western blot - Anti-Apo-D antibody [EPR22967-72] (ab256496)

All lanes : Anti-Apo-D antibody [EPR22967-72] (ab256496) at 1/1000 dilution

Lane 1 : Human plasma
Lane 2 : Human heart tissue lysate
Lane 3 : Human kidney tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 21 kDa
Observed band size: 22-35 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The smeared bands between 22-35 kDa are mainly caused by different levels of glycosylation (PMID: 26829325).

Exposure time: Lane 1: 26 seconds; Lanes 2-3: 3 minutes.
Immunoprecipitation - Anti-Apo-D antibody [EPR22967-72] (ab256496)

Apo-D was immunoprecipitated from 0.35 mg Human placenta lysate with ab256496 at 1/40 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256496 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: Human placenta lysate 10ug

Lane 2: ab256496 IP in Human placenta lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256496 in Human placenta lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.
Immunoprecipitation - Anti-Apo-D antibody [EPR22967-72] (ab256496)

Apo-D was immunoprecipitated from 0.35 mg Human plasma lysate with ab256496 at 1/40 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256496 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: Human plasma lysate 10ug

Lane 2: ab256496 IP in Human plasma lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256496 in Human plasma lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 10 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR22967-72] (ab256496)

Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling Apo-D with ab256496 at 1/2000 dilution (0.35ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human breast cancer (PMID:18330697, 18505051). Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR22967-72] (ab256496)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Apo-D with ab256496 at 1/2000 dilution (0.35ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in stromal cells of human colon (PMID: 23296401). Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Anti-Apo-D antibody [EPR22967-72] (ab256496)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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