Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Apo-D antibody [EPR2916] (ab108191)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2916] to Apo-D
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Apo-D antibody [EPR2916]
    See all Apo-D primary antibodies

  • Description

    Rabbit monoclonal [EPR2916] to Apo-D

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human plasma cell lysate IHC-P: Human kidney or liver tissue

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Previously labelled as Apolipoprotein D. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Apo-D antibody [EPR2916] (ab108191)
    Western blot - Anti-Apo-D antibody [EPR2916] (ab108191)
    Anti-Apo-D antibody [EPR2916] (ab108191) at 1/2000 dilution (purified) + human plasma at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size: 21 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] (ab108191)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] (ab108191)
    Immunohistochemical staining of paraffin embedded human liver with purified ab108191 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Western blot - Anti-Apo-D antibody [EPR2916] (ab108191)
    Western blot - Anti-Apo-D antibody [EPR2916] (ab108191)
    Anti-Apo-D antibody [EPR2916] (ab108191) at 1/1000 dilution (unpurified) + Human plasma cell lysate at 10 µg

    Predicted band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] (ab108191)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] (ab108191)

    Immunohistochemical staining of Apo-D in paraffin embedded human kidney tissue, using unpurified ab108191 at a 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] (ab108191)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] (ab108191)

    Immunohistochemical staining of Apo-D in paraffin embedded Human liver tissue, using unpurified ab108191 at a 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-Apo-D antibody [EPR2916] (ab108191)
    Anti-Apo-D antibody [EPR2916] (ab108191)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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