Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Apo-D antibody [EPR22967-72] - BSA and Azide free (ab261911)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22967-72] to Apo-D - BSA and Azide free
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Apo-D antibody [EPR22967-72] - BSA and Azide free
    See all Apo-D primary antibodies

  • Description

    Rabbit monoclonal [EPR22967-72] to Apo-D - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human plasma, Human heart and Human kidney lysates. IHC-P: Human colon and Human breast cancer tissues. IP: Human plasma and Human placenta cells.

  • General notes

    Ab261911 is the carrier-free version of ab256496. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab261911 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-Apo-D antibody [EPR22967-72] - BSA and Azide free (ab261911)
    Immunoprecipitation - Anti-Apo-D antibody [EPR22967-72] - BSA and Azide free (ab261911)

    Apo-D was immunoprecipitated from 0.35 mg Human placenta lysate with ab256496 at 1/40 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256496 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: Human placenta lysate 10ug

    Lane 2: ab256496 IP in Human placenta lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256496 in Human placenta lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256496).

  • Immunoprecipitation - Anti-Apo-D antibody [EPR22967-72] - BSA and Azide free (ab261911)
    Immunoprecipitation - Anti-Apo-D antibody [EPR22967-72] - BSA and Azide free (ab261911)

    Apo-D was immunoprecipitated from 0.35 mg Human plasma lysate with ab256496 at 1/40 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256496 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: Human plasma lysate 10ug

    Lane 2: ab256496 IP in Human plasma lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256496 in Human plasma lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256496).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR22967-72] - BSA and Azide free (ab261911)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR22967-72] - BSA and Azide free (ab261911)

    Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling Apo-D with ab256496 at 1/2000 dilution (0.35ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human breast cancer (PMID:18330697, 18505051). Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256496).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR22967-72] - BSA and Azide free (ab261911)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR22967-72] - BSA and Azide free (ab261911)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Apo-D with ab256496 at 1/2000 dilution (0.35ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in stromal cells of human colon (PMID: 23296401). Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256496).

  • Anti-Apo-D antibody [EPR22967-72] - BSA and Azide free (ab261911)
    Anti-Apo-D antibody [EPR22967-72] - BSA and Azide free (ab261911)

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