Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Apo-D antibody [EPR2916] - BSA and Azide free (ab247602)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2916] to Apo-D - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Apo-D antibody [EPR2916] - BSA and Azide free
    See all Apo-D primary antibodies

  • Description

    Rabbit monoclonal [EPR2916] to Apo-D - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247602 is the carrier-free version of ab108191 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247602 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Apo-D antibody [EPR2916] - BSA and Azide free (ab247602)
    Western blot - Anti-Apo-D antibody [EPR2916] - BSA and Azide free (ab247602)
    Anti-Apo-D antibody [EPR2916] (ab108191) at 1/2000 dilution (purified) + human plasma at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size: 21 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab108191, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] - BSA and Azide free (ab247602)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] - BSA and Azide free (ab247602)
    This data was developed using ab108191, the same antibody clone in a different buffer formulation.Immunohistochemical staining of paraffin embedded human liver with purified ab108191 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Western blot - Anti-Apo-D antibody [EPR2916] - BSA and Azide free (ab247602)
    Western blot - Anti-Apo-D antibody [EPR2916] - BSA and Azide free (ab247602)
    Anti-Apo-D antibody [EPR2916] (ab108191) at 1/1000 dilution (unpurified) + Human plasma at 10 µg

    Predicted band size: 21 kDa



    This data was developed using ab108191, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] - BSA and Azide free (ab247602)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] - BSA and Azide free (ab247602)
    This data was developed using ab108191, the same antibody clone in a different buffer formulation.Immunohistochemical staining of Apo-D in paraffin embedded human kidney tissue, using unpurified ab108191 at a 1/100 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] - BSA and Azide free (ab247602)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apo-D antibody [EPR2916] - BSA and Azide free (ab247602)
    This data was developed using ab108191, the same antibody clone in a different buffer formulation.Immunohistochemical staining of Apo-D in paraffin embedded Human liver tissue, using unpurified ab108191 at a 1/100 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Anti-Apo-D antibody [EPR2916] - BSA and Azide free (ab247602)
    Anti-Apo-D antibody [EPR2916] - BSA and Azide free (ab247602)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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