Anti-RRM1 antibody (ab226391)
Key features and details
- Rabbit polyclonal to RRM1
- Suitable for: IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RRM1 antibody
See all RRM1 primary antibodies -
Description
Rabbit polyclonal to RRM1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
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Immunogen
Synthetic peptide within Human RRM1 aa 742-792. The exact sequence is proprietary.
Database link: P23921
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-RRM1 antibody (ab226391)All lanes : Anti-RRM1 antibody (ab226391) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 30 seconds
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Immunoprecipitation - Anti-RRM1 antibody (ab226391)RRM1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN buffer: 1.0 mg for IP, 20% of IP loaded) with ab226389 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226391 at 0.4 µg/ml.
Lane 1: ab226391 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
