Rat IL-13 ELISA Kit (ab269547)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 20.03 pg/ml
- Range: 78.13 pg/ml - 5000 pg/ml
- Sample type: Cell culture media, EDTA Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat IL-13 ELISA Kit
See all IL-13 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 5.9% Inter-assay Sample n Mean SD CV% Serum 3 2% -
Sample type
Urine, Serum, Cell culture media, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
20.03 pg/ml -
Range
78.13 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 83 80% - 86% Serum 87 81% - 92% Cell culture media 90 84% - 98% EDTA Plasma 97 84% - 117% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Rat -
Product overview
Rat IL-13 ELISA Kit (ab269547) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-13 protein in edta plasma, serum, urine, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat IL-13 with 20.03 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Interleukin-13 (IL-13) is a secreted immunoregulatory cytokine in the IL-4/IL-13 family that is linked to allergic reactions and cancer pathogenesis. IL-13 inhibits inflammatory cytokine production and promotes immunoglobulin class switching on B-cells. IL-13 synergizes with IL-2 in regulating interferon-gamma synthesis. IL-13 is linked to allergic rhinitis, a common disease of complex inheritance characterized by mucosal inflammation caused by allergen exposure. The antibodies for this kit were raised against a recombinant peptide fragment of the mature rat protein.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Rat IL-13 Capture Antibody 1 x 600µl 10X Rat IL-13 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Plate Seals 1 unit Rat IL-13 Lyophilized Recombinant Protein 2 vials Sample Diluent 50BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages. -
Involvement in disease
Allergic rhinitis -
Sequence similarities
Belongs to the IL-4/IL-13 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Allergic rhinitis
- ALRH
- BHR 1
see all -
Database links
- Entrez Gene: 116553 Rat
- SwissProt: P42203 Rat
- Unigene: 9921 Rat
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of rat IL-13 standard curve in Sample Diluent 50BP.The IL-13 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spiked recombinant IL-13 in rat serum, plasma, urine and cell culture media samples.The concentrations of IL-13 were measured in duplicates, interpolated from the IL-13 standard curves and corrected for sample dilution. Undiluted samples are as follows: rat serum 50%, rat plasma (EDTA) 50%, rat urine 50% and cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Sandwich ELISA - Rat IL-13 ELISA Kit (ab269547)To learn more about the advantages of recombinant antibodies see here.
