Anti-Surfactant Protein A/PSAP antibody (ab87674)
Key features and details
- Rabbit polyclonal to Surfactant Protein A/PSAP
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Surfactant Protein A/PSAP antibody
See all Surfactant Protein A/PSAP primary antibodies -
Description
Rabbit polyclonal to Surfactant Protein A/PSAP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human Surfactant Protein A/PSAP aa 200 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab87829) -
Positive control
- This antibody gave a positive signal in Human Lung Tissue Lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human Lung.
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General notes
This product was previously labelled as Surfactant Protein A
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab87674 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species IHC-P HumanWB HumanAll applications PigNon human primatesApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 26 kDa).IHC-P Use a concentration of 0.1 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 26 kDa).IHC-P
Use a concentration of 0.1 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.Target
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Cellular localization
Secreted protein; extracellular space. -
Database links
- Entrez Gene: 6435 Human
- Entrez Gene: 6436 Human
- Entrez Gene: 397503 Pig
- Omim: 178630 Human
- Omim: 178642 Human
- SwissProt: Q8IWL1 Human
- SwissProt: Q8IWL2 Human
- SwissProt: P49874 Pig
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Alternative names
- 35 kDa pulmonary surfactant-associated protein antibody
- Alveolar proteinosis protein antibody
- COLEC4 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody (ab87674)
IHC image of Protein Surfactant A/PSAP staining in a section of formalin-fixed paraffin-embedded normal human lung performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab87674, 0.1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-Surfactant Protein A/PSAP antibody (ab87674)Anti-Surfactant Protein A/PSAP antibody (ab87674) at 1 µg/ml + Lung (Human) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutesSurfactant Protein A/PSAP contains a number of potential glycosylation sites and other post translational modifications (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Protocols
Datasheets and documents
References (1)
ab87674 has been referenced in 1 publication.
- Stoeckelhuber M et al. Immunolocalization of Surfactant Proteins SP-A, SP-B, SP-C, and SP-D in Infantile Labial Glands and Mucosa. J Histochem Cytochem 66:531-538 (2018). PubMed: 29601229
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Surfactant Protein A/PSAP antibody (ab87674)
IHC image of Protein Surfactant A/PSAP staining in a section of formalin-fixed paraffin-embedded normal human lung performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab87674, 0.1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-Surfactant Protein A/PSAP antibody (ab87674)Anti-Surfactant Protein A/PSAP antibody (ab87674) at 1 µg/ml + Lung (Human) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutesSurfactant Protein A/PSAP contains a number of potential glycosylation sites and other post translational modifications (SwissProt) which may explain its migration at a higher molecular weight than predicted.
