Recombinant rat PDE7B protein (ab198460)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant rat PDE7B protein
See all PDE7B proteins and peptides -
Biological activity
Specific Activity: ≥324 pmol/min/µg.
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.05% Tween-20, 0.1 mg/ml BSA, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of ab198460 at 37°C for 20 min. Quantified by 5'-nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. -
Purity
30 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Rat -
Sequence
MLSKVGMWDFDIFLFDRLTNGNSLVTLLCHLFNSHGLIHHFKLDMVTLHR FLVMVQEDYHGHNPYHNAVHAADVTQAMHCYLKEPKLASFLTPLDIMLGL LAAAAHDVDHPGVNQPFLIKTNHHLANLYQNMSVLENHHWRSTIGMLRES RLLAHLPKEMTQDIEQQLGSLILATDINRQNEFLTRLKAHLHNKDLRLEN IQDRHFMLQIALKCADICNPCRIWEMSKQWSERVCEEFYRQGDLEQKFEL EISPLCNQQKDSIPSIQIGFMTYIVEPLFREWARFTGNSTLSESMLNHLA HNKAQWKSLLSNQHRRRGSGQDPAGTAPETLEQTEGATP -
Predicted molecular weight
65 kDa including tags -
Amino acids
108 to 446 -
Tags
GST tag N-Terminus -
Additional sequence information
NM_080894.
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.71% Tris HCl, 0.72% Sodium chloride, 0.02% Potassium chloride, 0.55% Glutathione, 10% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant rat PDE7B protein (ab198460)
10% SDS-PAGE analysis of ab198460 with Coomassie staining.
Lane 1: 4 µg ab198460
Lane 2: Protein marker -
Functional Studies - Recombinant rat PDE7B protein (ab198460)Specific activity of ab198460.
