Specific Activity: ≥324 pmol/min/µg.
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.05% Tween-20, 0.1 mg/ml BSA, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of ab198460 at 37°C for 20 min. Quantified by 5'-nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

pH: 8.00
Constituents: 0.71% Tris HCl, 0.72% Sodium chloride, 0.02% Potassium chloride, 0.55% Glutathione, 10% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

This product is an active protein and may elicit a biological response in vivo, handle with caution.