Recombinant mouse PDE7B protein (ab198479)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 65% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant mouse PDE7B protein
See all PDE7B proteins and peptides -
Biological activity
Specific Activity: ≥240 pmol/min/µg.
Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.05% Tween-20, 0.1 mg/ml BSA, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of ab198479 at 37°C for 20 min. Quantified by 5'-nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
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Purity
>= 65 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
MLSKVGTWDFDIFLFDRLTNGNSLVTLLCHLFNSHGLIHHFKLDMVTLHR FLVMVQEDYHGHNPYHNAVHAADVTQAMHCYLKEPKLASFLTPLDIMLGL LAAAAHDVDHPGVNQPFLIKTNHHLANLYQNMSVLENHHWRSTIGMLRES RLLAHLPKEMTQDIEQQLGSLILATDINRQNEFLTRLKAHLHNKDLRLEN VQDRHFMLQIALKCADICNPCRIWEMSKQWSERVCEEFYRQGDLEQKFEL EISPLCNQQKDSIPSIQIGFMTYIVEPLFREWARFTGNSTLSENMLSHLA HNKAQWKSLLSNQHRRRGSGQDLAGPAPETLEQTEGATP -
Predicted molecular weight
65 kDa including tags -
Amino acids
108 to 446 -
Tags
GST tag N-Terminus
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Preparation and Storage
- bA472E5.1
- cAMP specific 3 5 cyclic phosphodiesterase 7B
- cAMP specific 3'5' cyclic phosphodiesterase 7B
Images
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SDS-PAGE - Recombinant mouse PDE7B protein (ab198479)
10% SDS-PAGE analysis of ab198479 with Coomassie staining.
Lane 1: 10 μg ab198479
Lane 2: Protein marker -
Functional Studies - Recombinant mouse PDE7B protein (ab198479)Example of specific activity of ab198479
