Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SLP76 (phospho Y145) antibody [EP2853Y] - BSA and Azide free (ab206782)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2853Y] to SLP76 (phospho Y145) - BSA and Azide free
  • Suitable for: ICC, WB, Flow Cyt, Dot blot
  • Reacts with: Human

Overview

  • Product name

    Anti-SLP76 (phospho Y145) antibody [EP2853Y] - BSA and Azide free
    See all SLP76 primary antibodies

  • Description

    Rabbit monoclonal [EP2853Y] to SLP76 (phospho Y145) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody detects SLP76 phosphorylated on tyrosine 145.

  • Tested applications

    Suitable for: ICC, WB, Flow Cyt, Dot blotmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Jurkat cell lysates treated with pervanadate.

  • General notes

    ab206782 is the carrier-free version of ab75829 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab206782 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EP2853Y

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-SLP76 (phospho Y145) antibody [EP2853Y] - BSA and Azide free (ab206782)
    Western blot - Anti-SLP76 (phospho Y145) antibody [EP2853Y] - BSA and Azide free (ab206782)
    All lanes : Anti-SLP76 (phospho Y145) antibody [EP2853Y] (ab75829) at 1/5000 dilution

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
    Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates. treated with pervanadate at 50nM for 5 min.
    Lane 3 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates. Treated with pervanadate at 50nM for 5 min. Then the membrane was incubated with phosphatase.

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 60 kDa
    Observed band size: 76 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab75829, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-SLP76 (phospho Y145) antibody [EP2853Y] - BSA and Azide free (ab206782)
    Immunocytochemistry - Anti-SLP76 (phospho Y145) antibody [EP2853Y] - BSA and Azide free (ab206782)
    This data was developed using ab75829, the same antibody clone in a different buffer formulation.ab75829 staining SLP76 in Jurkat by Immunocytochemistry. Tissue was fixed with 4% paraformaldehyde. Samples were incubated with primary antibody (4.5 μg/ml). ab150077 AlexaFluor®488 Goat anti-Rabbit (1/1000) was used as the secondary antibody. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 2.5 μg/ml and DAPI were used as counter stains. Confocal image showing the expression was increased after treatment with pervanadate (1mM, 30min) and then decreased after treatment with the Lambda Protein Phosphatase 31? for 2h
  • Immunocytochemistry - Anti-SLP76 (phospho Y145) antibody [EP2853Y] - BSA and Azide free (ab206782)
    Immunocytochemistry - Anti-SLP76 (phospho Y145) antibody [EP2853Y] - BSA and Azide free (ab206782)
    This data was developed using ab75829, the same antibody clone in a different buffer formulation.ab75829 staining SLP76 (phospho Y145) antibody in both treated (50mM of pervanadate for 5 minutes) and untreated Jurkat (human acute T cell leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.
  • Western blot - Anti-SLP76 (phospho Y145) antibody [EP2853Y] - BSA and Azide free (ab206782)
    Western blot - Anti-SLP76 (phospho Y145) antibody [EP2853Y] - BSA and Azide free (ab206782)
    All lanes : Anti-SLP76 (phospho Y145) antibody [EP2853Y] (ab75829) at 1/200000 dilution

    Lane 1 : Jurkat cell lysate un-treated
    Lane 2 : Jurkat cell lysate treated with pervanadate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 60 kDa
    Observed band size: 76 kDa why is the actual band size different from the predicted?



    This data was developed using ab75829, the same antibody clone in a different buffer formulation.

  • Dot Blot - Anti-SLP76 (phospho Y145) antibody [EP2853Y] - BSA and Azide free (ab206782)
    Dot Blot - Anti-SLP76 (phospho Y145) antibody [EP2853Y] - BSA and Azide free (ab206782)
    This data was developed using ab75829, the same antibody clone in a different buffer formulation.Dot blot analysis of SLP76 (phospho Y145) phospho peptide (Lane 1), SLP76 Non-phospho peptide (Lane 2), labelling SLP76 (phospho Y145) with ab75829 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/2500.Blocking and diluting buffer: 5% NFDM/TBST.Exposure time: 3 minutes.
  • Flow Cytometry - Anti-SLP76 (phospho Y145) antibody [EP2853Y] - BSA and Azide free (ab206782)
    Flow Cytometry - Anti-SLP76 (phospho Y145) antibody [EP2853Y] - BSA and Azide free (ab206782)
    This data was developed using ab75829, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized Jurkat cells, untreated (green) or pervanadate treated (red) using ab75829 at a 1/100 dilution. Also, pervanadate Jurkat cells using the same antibody preincubated with phospho SLP76 peptide (blue) or non phospho SLP76 peptide (orange).
  • Anti-SLP76 (phospho Y145) antibody [EP2853Y] - BSA and Azide free (ab206782)
    Anti-SLP76 (phospho Y145) antibody [EP2853Y] - BSA and Azide free (ab206782)

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