Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IL-28A + IL-28B antibody [EPR5328(2)] - BSA and Azide free (ab248141)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5328(2)] to IL-28A + IL-28B - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Recombinant fragment

Overview

  • Product name

    Anti-IL-28A + IL-28B antibody [EPR5328(2)] - BSA and Azide free

  • Description

    Rabbit monoclonal [EPR5328(2)] to IL-28A + IL-28B - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Recombinant fragment
    Predicted to work with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human IL-28A and IL-28B recombinant protein

  • General notes

    Ab248141 is the carrier-free version of ab126713. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248141 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-IL-28A + IL-28B antibody [EPR5328(2)] - BSA and Azide free (ab248141)
    Western blot - Anti-IL-28A + IL-28B antibody [EPR5328(2)] - BSA and Azide free (ab248141)
    All lanes : Anti-IL-28A + IL-28B antibody [EPR5328(2)] (ab126713) at 1/1000 dilution

    Lane 1 : Human IL-28A recombinant protein (aa 27 to 200)
    Lane 2 : House made His-tagged full length human IL-28B recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 22 kDa



    Exposure time

    Lane 1: 40 seconds

    Lane 2: 3 second

    Buffer and concentration: 5% NFDM /TBST

  • Western blot - Anti-IL-28A + IL-28B antibody [EPR5328(2)] - BSA and Azide free (ab248141)
    Western blot - Anti-IL-28A + IL-28B antibody [EPR5328(2)] - BSA and Azide free (ab248141)
    Anti-IL-28A + IL-28B antibody [EPR5328(2)] (ab126713) at 1/5000 dilution + Human IL-28A (amino acids 27-200) recombinant protein

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 22 kDa
    Observed band size: 19 kDa
    why is the actual band size different from the predicted?


    Exposure time: 120 seconds


    This data was developed using ab126713, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-IL-28A + IL-28B antibody [EPR5328(2)] - BSA and Azide free (ab248141)
    Anti-IL-28A + IL-28B antibody [EPR5328(2)] - BSA and Azide free (ab248141)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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