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Signal Transduction Signaling Pathway Nuclear Signaling NFkB Pathway

Anti-NFkB p100/NFKB2 antibody [EPR18756] - BSA and Azide free (ab251092)

Anti-NFkB p100/NFKB2 antibody [EPR18756] - BSA and Azide free (ab251092)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18756] to NFkB p100/NFKB2 - BSA and Azide free
  • Suitable for: Flow Cyt, ICC, IP, WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-NFkB p100/NFKB2 antibody [EPR18756] - BSA and Azide free
    See all NFkB p100/NFKB2 primary antibodies
  • Description

    Rabbit monoclonal [EPR18756] to NFkB p100/NFKB2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, ICC, IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251092 is the carrier-free version of ab191594 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab251092 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as NFkB p100

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    EPR18756
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

Images

  • Western blot - Anti-NFkB p100/NFKB2 antibody [EPR18756] - BSA and Azide free (ab251092)
    Western blot - Anti-NFkB p100/NFKB2 antibody [EPR18756] - BSA and Azide free (ab251092)
    All lanes : Anti-NFkB p100/NFKB2 antibody [EPR18756] (ab191594) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 97 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    This data was developed using ab191594, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what have been described in the literature (PMID: 8606850, 17015635).

  • Western blot - Anti-NFkB p100/NFKB2 antibody [EPR18756] - BSA and Azide free (ab251092)
    Western blot - Anti-NFkB p100/NFKB2 antibody [EPR18756] - BSA and Azide free (ab251092)
    All lanes : Anti-NFkB p100/NFKB2 antibody [EPR18756] (ab191594) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 4 : Daudi (Human Burkitt's lymphoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 97 kDa
    Observed band size: 110 kDa why is the actual band size different from the predicted?



    This data was developed using ab191594, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-3: 30 seconds; Lane 4: 5 seconds.

    The molecular weight observed is consistent with what have been described in the literature (PMID: 18606850, 17015635).

  • Western blot - Anti-NFkB p100/NFKB2 antibody [EPR18756] - BSA and Azide free (ab251092)
    Western blot - Anti-NFkB p100/NFKB2 antibody [EPR18756] - BSA and Azide free (ab251092)
    All lanes : Anti-NFkB p100/NFKB2 antibody [EPR18756] (ab191594) at 1/1000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Rat brain lysate
    Lane 4 : Rat heart lysate
    Lane 5 : Rat kidney lysate
    Lane 6 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 97 kDa
    Observed band size: 110 kDa why is the actual band size different from the predicted?



    This data was developed using ab191594, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-2: 3 minutes; Lanes 3-4: 30 seconds; Lanes 5-6: 5 seconds.

    The molecular weight observed is consistent with what have been described in the literature (PMID: 18606850, 17015635).

  • Western blot - Anti-NFkB p100/NFKB2 antibody [EPR18756] - BSA and Azide free (ab251092)
    Western blot - Anti-NFkB p100/NFKB2 antibody [EPR18756] - BSA and Azide free (ab251092)
    All lanes : Anti-NFkB p100/NFKB2 antibody [EPR18756] (ab191594) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embyro fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 97 kDa
    Observed band size: 110 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    This data was developed using ab191594, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what have been described in the literature (PMID: 18606850, 17015635).

  • Immunocytochemistry - Anti-NFkB p100/NFKB2 antibody [EPR18756] - BSA and Azide free (ab251092)
    Immunocytochemistry - Anti-NFkB p100/NFKB2 antibody [EPR18756] - BSA and Azide free (ab251092)
    This data was developed using ab191594, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cell line labeling NFkB p100/NFKB2 with ab191594 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red). The negative controls are as follows:- -ve control 1: ab191594 at 1/250 dilution followed by ab150120 at 1/1000 dilution. -ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
  • Immunocytochemistry - Anti-NFkB p100/NFKB2 antibody [EPR18756] - BSA and Azide free (ab251092)
    Immunocytochemistry - Anti-NFkB p100/NFKB2 antibody [EPR18756] - BSA and Azide free (ab251092)
    This data was developed using ab191594, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embyro fibroblast cell line) cell line labeling NFkB p100/NFKB2 with ab191594 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on NIH/3T3 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red). The negative controls are as follows:- -ve control 1: ab191594 at 1/250 dilution followed by ab150120 at 1/1000 dilution. -ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
  • Flow Cytometry - Anti-NFkB p100/NFKB2 antibody [EPR18756] - BSA and Azide free (ab251092)
    Flow Cytometry - Anti-NFkB p100/NFKB2 antibody [EPR18756] - BSA and Azide free (ab251092)
    This data was developed using ab191594, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cell line labeling NFkB p100/NFKB2 with ab191594 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.
  • Immunoprecipitation - Anti-NFkB p100/NFKB2 antibody [EPR18756] - BSA and Azide free (ab251092)
    Immunoprecipitation - Anti-NFkB p100/NFKB2 antibody [EPR18756] - BSA and Azide free (ab251092)

    This data was developed using ab191594, the same antibody clone in a different buffer formulation.

    NFkB p100/NFKB2 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab191594 at 1/20 dilution.

    Western blot was performed from the immunoprecipitate using ab191594 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/10000 dilution.

    Lane 1: HeLa whole cell lysate, 10ug (Input).

    Lane 2: ab191594 IP in HeLa whole cell lysate.

    Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab191594 in HeLa whole cell lysate.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 seconds.

  • Anti-NFkB p100/NFKB2 antibody [EPR18756] - BSA and Azide free (ab251092)
    Anti-NFkB p100/NFKB2 antibody [EPR18756] - BSA and Azide free (ab251092)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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