Anti-Granulin antibody [8H10] (ab252834)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [8H10] to Granulin
- Suitable for: WB
- Reacts with: Mouse
Overview
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Product name
Anti-Granulin antibody [8H10]
See all Granulin primary antibodies -
Description
Rat monoclonal [8H10] to Granulin -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: J774A.1 whole cell lysate treated and not treated with PNGase F. Hepa1-6, 4T1, RAW264.7 and NIH/3T3 whole cell lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
8H10 -
Isotype
IgG2c -
Research areas
Images
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All lanes : Anti-Granulin antibody [8H10] (ab252834) at 1.14 µg/ml
Lane 1 : Hepa1-6 (mouse hepatoma epithelial cell), whole cell lysate
Lane 2 : 4T1 (mouse mammary gland carcinoma epithelial cell), whole cell lysate
Lane 3 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lane 4 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution
Predicted band size: 64 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Granulin is a glycoprotein, the molecular weight observed is consistent with what has been described in the literature (PMID: 8496151).
Exposure times: Lanes 1-2/4: 37 seconds; Lanes 3: 5 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Granulin antibody [8H10] (ab252834) at 1.14 µg/ml
Lane 1 : J774A.1 (mouse reticulum cell sarcoma monocyte macrophage), whole cell lysate
Lane 2 : J774A.1 treated with PNGase F whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution
Predicted band size: 64 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 136 secondsGranulin is a glycoprotein, the molecular weight observed is consistent with what has been described in the literature (PMID: 8496151).
Blocking/Dilution buffer: 5% NFDM/TBST.
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