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Signal Transduction Protein Trafficking Vesicle Transport Regulation

Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202)

Price and availability

278 083 ₸

Availability

Order now and get it on Wednesday March 24, 2021

Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [4B3-G7-H5] to EEF2/Elongation factor 2
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Human, African green monkey
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5]
    See all EEF2/Elongation factor 2 primary antibodies
  • Description

    Mouse monoclonal [4B3-G7-H5] to EEF2/Elongation factor 2
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, African green monkey
  • Immunogen

    Recombinant fragment corresponding to Human EEF2/Elongation factor 2.
    Database link: NM_001961.3

  • Positive control

    • HL-60 cell lysate
  • General notes

    This product was changed from ascites to tissue culture supernatant on 17.08.2018.  Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

     This product was previously labelled as EEF2

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin 300
    Constituent: 50% Glycerol
  • Concentration information loading...
  • Purity

    Concentrated Culture Supernatant
  • Clonality

    Monoclonal
  • Clone number

    4B3-G7-H5
  • Isotype

    IgG2b
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation

Images

  • Western blot - Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202)
    Western blot - Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202)
    All lanes : Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202) at 1/5000 dilution

    Lane 1 : Jurkat Cell lysates
    Lane 2 : C6 Cell lysates
    Lane 3 : 3T3 Cell lysates
    Lane 4 : COS-7 (African green monkey kidney fibroblast-like cell line) Cell lysates
    Lane 5 : HeLa Cell lysates

    Predicted band size: 95 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202)
    Immunocytochemistry/ Immunofluorescence - Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202)

    Immunocytochemical staining of HeLa cells using anti-eEF2/Elongation factor 2 antibody (ab131202) at a dilution of 1:200. Cells were fixed with ice cold methanol. This image was produced with antibody produced in ascites. 

  • Western blot - Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202)
    Western blot - Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202)

    Western blot detection of EEF2/Elongation factor 2 in HL-60,Jurkat,SHSY-5Y,U20S and Hela cell lysates using EEF2/Elongation factor 2 mouse mAb (1:5000).Predicted band size:95KDa.Observed band size: 95KDa.Exposure time:15s. This image was produced with antibody produced in ascites. 

  • Western blot - Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202)
    Western blot - Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202)
    Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202) at 1/1000 dilution + HL-60 cell lysate

    Predicted band size: 95 kDa
    Observed band size: 95 kDa



    This image was produced with antibody produced in ascites. 

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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