Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202)
Key features and details
- Mouse monoclonal [4B3-G7-H5] to EEF2/Elongation factor 2
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG2b
Overview
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Product name
Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5]
See all EEF2/Elongation factor 2 primary antibodies -
Description
Mouse monoclonal [4B3-G7-H5] to EEF2/Elongation factor 2 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, African green monkey -
Immunogen
Recombinant fragment corresponding to Human EEF2/Elongation factor 2.
Database link: NM_001961.3 -
Positive control
- HL-60 cell lysate
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General notes
This product was changed from ascites to tissue culture supernatant on 17.08.2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
This product was previously labelled as EEF2
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituent: 50% Glycerol -
Concentration information loading...
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Purity
Concentrated Culture Supernatant -
Clonality
Monoclonal -
Clone number
4B3-G7-H5 -
Isotype
IgG2b -
Research areas
Images
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All lanes : Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202) at 1/5000 dilution
Lane 1 : Jurkat Cell lysates
Lane 2 : C6 Cell lysates
Lane 3 : 3T3 Cell lysates
Lane 4 : COS-7 (African green monkey kidney fibroblast-like cell line) Cell lysates
Lane 5 : HeLa Cell lysates
Predicted band size: 95 kDa
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Immunocytochemical staining of HeLa cells using anti-eEF2/Elongation factor 2 antibody (ab131202) at a dilution of 1:200. Cells were fixed with ice cold methanol. This image was produced with antibody produced in ascites.
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Western blot detection of EEF2/Elongation factor 2 in HL-60,Jurkat,SHSY-5Y,U20S and Hela cell lysates using EEF2/Elongation factor 2 mouse mAb (1:5000).Predicted band size:95KDa.Observed band size: 95KDa.Exposure time:15s. This image was produced with antibody produced in ascites.
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Anti-EEF2/Elongation factor 2 antibody [4B3-G7-H5] (ab131202) at 1/1000 dilution + HL-60 cell lysate
Predicted band size: 95 kDa
Observed band size: 95 kDaThis image was produced with antibody produced in ascites.