Anti-ADK antibody (ab88903)
Key features and details
- Rabbit polyclonal to ADK
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-ADK antibody
See all ADK primary antibodies -
Description
Rabbit polyclonal to ADK -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species sELISA HumanWB Human
-
Immunogen
Synthetic peptide corresponding to Human ADK aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available asab101315) -
Positive control
- This antibody gave a positive signal in the following lysates: RAW 264.7 Whole Cell Lysate; Human Placenta Tissue Lysate; Human Liver Tissue Lysate; HepG2 Whole Cell Lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88903 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species sELISA HumanWB HumanAll applications RatZebrafishChinese hamsterApplication Abreviews Notes WB Use at an assay dependent concentration. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).Notes WB
Use at an assay dependent concentration. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).Target
-
Function
ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides. -
Tissue specificity
Widely expressed. Highest level in placenta, liver, muscle and kidney. -
Pathway
Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine 3',5'-bisphosphate: step 1/1. -
Sequence similarities
Belongs to the carbohydrate kinase pfkB family. - Information by UniProt
-
Database links
- Entrez Gene: 100736551 Chinese hamster
- Entrez Gene: 132 Human
- Entrez Gene: 11534 Mouse
- Entrez Gene: 25368 Rat
- Omim: 102750 Human
- SwissProt: P55262 Chinese hamster
- SwissProt: P55263 Human
- SwissProt: P55264 Mouse
see all -
Alternative names
- 2310026J05Rik antibody
- 5033405D03Rik antibody
- Adenosine 5''-phosphotransferase antibody
see all
Images
-
Western blot - Anti-ADK antibody (ab88903)All lanes : Anti-ADK antibody (ab88903) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate at 40 µg
Lane 2 : ADK knockout HAP1 whole cell lysate at 40 µg
Lane 3 : HepG2 whole cell lysate at 20 µg
Predicted band size: 41 kDaLanes 1 - 3: Merged signal (red and green). Green - ab88903 observed at 41 kDa. Red - loading control, ab130007, observed at 130 kDa.
ab88903 was shown to recognize ADK in wild-type HAP1 cells as signal was lost at the expected MW in ADK knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ADK knockout samples were subjected to SDS-PAGE. Ab88903 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
Western blot - Anti-ADK antibody (ab88903)All lanes : Anti-ADK antibody (ab88903) at 1 µg/ml
Lane 1 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lane 2 : Human placenta tissue lysate - total protein (ab29745)
Lane 3 : Human liver tissue lysate - total protein (ab29889)
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 39,41 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes
Ab88903 is raised against an immunogen that is present in both isoforms of ADK. We hypothesise that the bands observed correspond to the long and short isoforms, which are 41- and 39-kDa respectively.
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab88903 has been referenced in 1 publication.
- Martínez-Vega R et al. Folic acid deficiency induces premature hearing loss through mechanisms involving cochlear oxidative stress and impairment of homocysteine metabolism. FASEB J 29:418-32 (2015). PubMed: 25384423
Images
-
Western blot - Anti-ADK antibody (ab88903)All lanes : Anti-ADK antibody (ab88903) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate at 40 µg
Lane 2 : ADK knockout HAP1 whole cell lysate at 40 µg
Lane 3 : HepG2 whole cell lysate at 20 µg
Predicted band size: 41 kDaLanes 1 - 3: Merged signal (red and green). Green - ab88903 observed at 41 kDa. Red - loading control, ab130007, observed at 130 kDa.
ab88903 was shown to recognize ADK in wild-type HAP1 cells as signal was lost at the expected MW in ADK knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ADK knockout samples were subjected to SDS-PAGE. Ab88903 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
Western blot - Anti-ADK antibody (ab88903)All lanes : Anti-ADK antibody (ab88903) at 1 µg/ml
Lane 1 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lane 2 : Human placenta tissue lysate - total protein (ab29745)
Lane 3 : Human liver tissue lysate - total protein (ab29889)
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 39,41 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes
Ab88903 is raised against an immunogen that is present in both isoforms of ADK. We hypothesise that the bands observed correspond to the long and short isoforms, which are 41- and 39-kDa respectively.
