Anti-TRPC6 antibody (ab101622)
Key features and details
- Rabbit polyclonal to TRPC6
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-TRPC6 antibody
See all TRPC6 primary antibodies -
Description
Rabbit polyclonal to TRPC6 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRat
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Immunogen
Synthetic peptide corresponding to Mouse TRPC6 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab116183) -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Mouse lung; Mouse kidney; Rat brain.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab101622 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseRatAll applications HorseCowApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 107 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 107 kDa).Target
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Function
Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion. -
Tissue specificity
Expressed primarily in placenta, lung, spleen, ovary and small intestine. Expressed in podocytes and is a component of the glomerular slit diaphragm. -
Involvement in disease
Defects in TRPC6 are the cause of focal segmental glomerulosclerosis type 2 (FSGS2) [MIM:603965]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. -
Sequence similarities
Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC6 sub-subfamily.
Contains 4 ANK repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 407151 Cow
- Entrez Gene: 22068 Mouse
- Entrez Gene: 89823 Rat
- SwissProt: Q61143 Mouse
- Unigene: 325086 Mouse
- Unigene: 105986 Rat
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Alternative names
- bZ1P14.9 antibody
- FLJ11098 antibody
- FLJ14863 antibody
see all
Images
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Western blot - Anti-TRPC6 antibody (ab101622)All lanes : Anti-TRPC6 antibody (ab101622) at 1 µg/ml
Lane 1 :Mouse lung normal tissue lysate - total protein (ab29297)
Lane 2 : Kidney (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 107 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
RPC6 contains a potential glycosylation site (SwissProt) which may explain the higher migration pattern observed. Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Datasheets and documents
References (1)
ab101622 has been referenced in 1 publication.
- Roa-Coria JE et al. Possible involvement of peripheral TRP channels in the hydrogen sulfide-induced hyperalgesia in diabetic rats. BMC Neurosci 20:1 (2019). PubMed: 30602386
Images
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Western blot - Anti-TRPC6 antibody (ab101622)All lanes : Anti-TRPC6 antibody (ab101622) at 1 µg/ml
Lane 1 :Mouse lung normal tissue lysate - total protein (ab29297)
Lane 2 : Kidney (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 107 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
RPC6 contains a potential glycosylation site (SwissProt) which may explain the higher migration pattern observed. Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
