Human Thyroxine ELISA Kit (fT4) (ab108662)
Key features and details
- Sensitivity: 0.5 ng/ml
- Sample type: Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Overview
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Product name
Human Thyroxine ELISA Kit (fT4)
See all Thyroxine (T4) kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Low 16 3pg/ml 0.03 9.8% Normal 16 14pg/ml 0.06 4.5% High 16 36pg/ml 0.22 6.2% Inter-assay Sample n Mean SD CV% Low 10 34pg/ml 0.04 11.5% Normal 10 13.5pg/ml 0.07 3.7% HIgh 10 36.9pg/ml 0.25 4.2% -
Sample type
Serum -
Assay type
Competitive -
Sensitivity
0.5 ng/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
ab108662, Thyroxine (fT4) Human ELISA Kit is intended for the quantitative determination of Free Thyroxine (fT4) concentration in Human serum.
Thyroxine (T4) or 3,5,3’,5’-tetraiodothyronine is the most commonly measured thyroid hormone for the diagnosis of thyroid function. Thyroxine (T4) has its primary infuence on protein synthesis and oxygen consumption in virtually all tissues but it is also important for growth, development, and sexual maturation. Thyroxine (T4) is synthesized by the thyroid gland and is secreted into the bloodstream. Here the Thyroxine (T4) becomes bound to serum proteins for transport to the cells. The major transport protein is Thyroxine Binding Globulin (TBG) which normally accounts for 80% of the bound Thyroxine (T4). Other thyroid hormone binding proteins are Thyroxine Binding Prealbumin and Albumin. Most of the serum Thyroxine (T4) is bound to these transport proteins leaving only about 0.03% free to exert its effect on cells. It is the free Thyroxine (T4) (fT4) that represents the metabolically active fraction; for this reason the measurement of fT4 concentration is considered to be an indicator of patient thyroid status. Primary hypothyroidism results in underproduction of Thyroxine (T4) by the thyroid gland and consequently an abnormally low circulating fT4 concentration in the blood. Primary hyperthyroidism leads to excessive thyroid production on Thyroxine (T4) and resulting elevated fT4 concentration.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests Color Reagent A 1 x 13ml Color Reagent B 1 x 13ml Free Thyroxine Standard 1 - 0ng/dL 1 x 1ml Free Thyroxine Standard 2 - 0.3 ng/dL 1 x 1ml Free Thyroxine Standard 3 - 0.95ng/dL 1 x 1ml Free Thyroxine Standard 4 - 2.1ng/dL 1 x 1ml Free Thyroxine Standard 5 - 3.6ng/dL 1 x 1ml Free Thyroxine Standard 6 - 7.0ng/dL 1 x 1ml Stop Solution 1 x 10ml T4 Antibody-Coated Microplate, 96 wells 1 x 96 packs Thyroxine (T4)-Enzyme Conjugate Reagent 1 x 10.5ml -
Research areas
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Database links
- SwissProt: P05543 Human
Images
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ELISA - Thyroxine (fT4) Human ELISA Kit (ab108662)Standard Curve
