Anti-Peptide YY/PYY antibody (ab22663)
Key features and details
- Rabbit polyclonal to Peptide YY/PYY
- Suitable for: IHC-FoFr, IHC-P
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-Peptide YY/PYY antibody
See all Peptide YY/PYY primary antibodies -
Description
Rabbit polyclonal to Peptide YY/PYY -
Host species
Rabbit -
Specificity
Immunostaining may be abolished by preincubation with 1nmol synthetic porcine PYY per mL diluted antibody. NPY and PP (10nmol/mL) have no effect on immunostaining. -
Tested applications
Suitable for: IHC-FoFr, IHC-Pmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Rabbit, Dog, Human
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Immunogen
Full length native protein (purified) corresponding to Pig Peptide YY/PYY aa 1-36.
Sequence:YPAKPEAPGE DASPEELSRY YASLRHYLNL VTRQRY
Database link: P68005 -
General notes
Previously labelled as Peptide YY.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.07% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (ab22663) Image courtesy of Carl Hobbs, Kings College London, U.K.
IHC image of Peptide YY/PYY staining in mouse colon formalin fixed paraffin embedded tissue section. Antigen retrieval was heat mediated with citrate and sections were blocked in 1% B.S.A for 10 minutes at 21ºC. Sample was incubated in primary antibody at 1/9000 for 2 hours and a biotin conjugated goat-anti-rabbit secondary was used at 1/250.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (ab22663) Image courtesy of Carl Hobbs, Kings College London, U.K.IHC image of Peptide YY/PYY staining in rat pancreas formalin fixed paraffin embedded tissue section. Antigen retrieval was heat mediated with citrate and sections were blocked in 1% B.S.A for 10 minutes at 21ºC. Sample was incubated in primary antibody at 1/9000 for 2 hours and a biotin conjugated goat-anti-rabbit secondary was used at 1/250.
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Peptide YY/PYY antibody (ab22663)Adjacent de-waxed sections of Bouin-fixed fetal (14 day) rat pancreas stained with rabbit polyclonal antisera to PYY (ab22663; panel a) and glucagon (ab18461; panel b). Arrows indicate identical cell profiles. From Ali-Rachedi et al, 1984. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (ab22663) Image from Acosta A et al., PLoS One. 2011;6(10):e26137. Epub 2011 Oct 10. Fig 2.; doi:10.1371/journal.pone.0026137; October 10, 2011, PLoS ONE 6(10): e26137.ab22663 staining Peptide YY/PYY (green) in murine pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue (left, WT; right, Peptide YY/PYY KO) was fixed with paraformaldehyde and blocked with 3% H2O2 in methanol, followed by antigen retrieval with trypsin. Samples were incubated with primary antibody (1/2000 in TNT) overnight. An AlexaFluor®488-conjugated donkey anti-rabbit IgG (1/1000 in TNT) was used as the secondary antibody. Nuclei were stained with DAPI (red).
