Human PYY ELISA Kit (ab255727)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 8.4 pg/ml
- Range: 15.63 pg/ml - 1000 pg/ml
- Sample type: EDTA Plasma, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human PYY ELISA Kit
See all Peptide YY/PYY kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% extract 8 11.7% Inter-assay Sample n Mean SD CV% extract 3 12.2% -
Sample type
Tissue Extracts, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
8.4 pg/ml -
Range
15.63 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Tissue Extracts 109 108% - 111% EDTA Plasma 92 81% - 106% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human PYY ELISA Kit (ab255727) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PYY protein in tissue extracts and edta plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PYY with 8.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Peptide PYY, also known as peptide tyrosine tyrosine, is a short 36- amino acid peptide encoded by the gene PYY. PYY is released from L cells in the ileum and colon in response to feeding, and functions by binding to NPY receptors to induce satiety. Two forms of PYY have been identified, PYY1-36 and PYY3-36, with the latter is most common form. PYY is cleaved at the Pro-30 residue by prolyl endopeptidase FAP(Separase) to generate peptide PYY3-36. PYY3-36 binds to the Y2 receptor of the Y family of receptors. PYY inhibits exocrine pancreatic secretion by vasoconstrictor action and inhibits jejunal/colonic mobility, allowing a more efficient digestion and nutrient absorption.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human PYY Capture Antibody 1 x 600µl 10X Human PYY Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human PYY Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility. -
Sequence similarities
Belongs to the NPY family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- GHYY
- MGC19143
- Peptide tyrosine tyrosine
see all -
Database links
- Entrez Gene: 5697 Human
- Omim: 600781 Human
- SwissProt: P10082 Human
- Unigene: 169249 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human PYY standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.The PYY standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native PYY in human small intestine and human colon extract.The concentrations of PYY were measured in duplicate and interpolated from the PYY standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Undiluted samples are as follows: small intestine extract 37.5 μg/mL and colon extract 150 μg/mL.
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Sandwich ELISA - Human PYY ELISA Kit (ab255727)To learn more about the advantages of recombinant antibodies see here.
