Anti-Peptide YY/PYY antibody (ab131246)
Key features and details
- Rabbit polyclonal to Peptide YY/PYY
- Suitable for: IHC-P
- Reacts with: Rat, Human, Common marmoset
- Isotype: IgG
Overview
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Product name
Anti-Peptide YY/PYY antibody
See all Peptide YY/PYY primary antibodies -
Description
Rabbit polyclonal to Peptide YY/PYY -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human, Common marmoset
Predicted to work with: Mouse, Dog, Pig, Macaque monkey, Chinese hamster
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Immunogen
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Positive control
- This antibody gave a positive signal in Rat normal Pancreas formalin fixed paraffin embedded tissue section.
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General notes
Previously labelled as Peptide YY.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab131246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Target
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Function
This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility. -
Sequence similarities
Belongs to the NPY family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 607156 Dog
- Entrez Gene: 5697 Human
- Entrez Gene: 217212 Mouse
- Entrez Gene: 445018 Pig
- Entrez Gene: 287730 Rat
- Omim: 600781 Human
- SwissProt: P68004 Dog
- SwissProt: P10082 Human
see all -
Alternative names
- GHYY antibody
- MGC19143 antibody
- Peptide tyrosine tyrosine antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (ab131246)Image courtesy of Carl Hobbs, Kings College London, U.K.
IHC image of Peptide YY/PYY staining in mouse pancreas formalin fixed paraffin embedded tissue section. Antigen retrieval was heat mediated with citrate and sections were blocked in 1% B.S.A for 10 minutes at 21ºC. Sample was incubated in primary antibody at 1/6000 for 2 hours and a biotin conjugated goat-anti-rabbit secondary was used at 1/250.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (ab131246)Image courtesy of Carl Hobbs, Kings College London, U.K.IHC image of Peptide YY/PYY staining in Human colon formalin fixed paraffin embedded tissue section. Antigen retrieval was heat mediated with citrate and sections were blocked in 1% B.S.A for 10 minutes at 21ºC. Sample was incubated in primary antibody at 1/8000 for 2 hours and a biotin conjugated goat-anti-rabbit secondary was used at 1/250.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (ab131246)Image Courtesy of Carl Hobbs, Kings College London, U.K.IHC image of Peptide YY/PYY staining in marmoset Jejunum formalin fixed paraffin embedded tissue section. Antigen retrieval was heat mediated with citrate and sections were blocked in 1% B.S.A for 10 minutes at 21ºC. Sample was incubated in primary antibody at 1/5000 for 2 hours and a biotin conjugated goat-anti-rabbit secondary was used at 1/250.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (ab131246)IHC image of Peptide YY/PYY staining in Rat normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab131246, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Datasheets and documents
References (2)
ab131246 has been referenced in 2 publications.
- Rodnoi P et al. Neuropeptide Y expression marks partially differentiated ß cells in mice and humans. JCI Insight 2:N/A (2017). ICC/IF ; Mouse . PubMed: 28614797
- Suzuki H & Yamamoto T Amylin-like immunoreactivity in pancreatic X cells of the black-spotted frog Rana (Pelophylax) nigromaculata. Tissue Cell 46:535-9 (2014). IHC-Fr ; Frog . PubMed: 25458814
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (ab131246) Image courtesy of Carl Hobbs, Kings College London, U.K.
IHC image of Peptide YY/PYY staining in mouse pancreas formalin fixed paraffin embedded tissue section. Antigen retrieval was heat mediated with citrate and sections were blocked in 1% B.S.A for 10 minutes at 21ºC. Sample was incubated in primary antibody at 1/6000 for 2 hours and a biotin conjugated goat-anti-rabbit secondary was used at 1/250.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (ab131246) Image courtesy of Carl Hobbs, Kings College London, U.K.
IHC image of Peptide YY/PYY staining in Human colon formalin fixed paraffin embedded tissue section. Antigen retrieval was heat mediated with citrate and sections were blocked in 1% B.S.A for 10 minutes at 21ºC. Sample was incubated in primary antibody at 1/8000 for 2 hours and a biotin conjugated goat-anti-rabbit secondary was used at 1/250.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (ab131246) Image Courtesy of Carl Hobbs, Kings College London, U.K.
IHC image of Peptide YY/PYY staining in marmoset Jejunum formalin fixed paraffin embedded tissue section. Antigen retrieval was heat mediated with citrate and sections were blocked in 1% B.S.A for 10 minutes at 21ºC. Sample was incubated in primary antibody at 1/5000 for 2 hours and a biotin conjugated goat-anti-rabbit secondary was used at 1/250.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (ab131246)
IHC image of Peptide YY/PYY staining in Rat normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab131246, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
