Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BAFF antibody [EPR22238] (ab224710)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22238] to BAFF
  • Suitable for: Flow Cyt, ICC/IF, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-BAFF antibody [EPR22238]
    See all BAFF primary antibodies

  • Description

    Rabbit monoclonal [EPR22238] to BAFF

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: U937 and HL-60 whole cell lysates. ICC/IF: U937 cells. Flow Cyt: HL-60 cells. IP: U937 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-BAFF antibody [EPR22238] (ab224710)
    Western blot - Anti-BAFF antibody [EPR22238] (ab224710)
    All lanes : Anti-BAFF antibody [EPR22238] (ab224710) at 1/1000 dilution

    Lane 1 : U937 (human histiocytic lymphoma cell line) whole cell lysate
    Lane 2 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate
    Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 4 : Daudi (human Burkitt's lymphoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 31 kDa
    Observed band size: 30,32 kDa
    why is the actual band size different from the predicted?


    Exposure time: 26 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-BAFF antibody [EPR22238] (ab224710)
    Immunoprecipitation - Anti-BAFF antibody [EPR22238] (ab224710)

    BAFF was immunoprecipitated from 0.35 mg of U937 (human histiocytic lymphoma cell line) whole cell lysate with ab224710 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab224710 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: U937 whole cell lysate 10 µg (Input). 

    Lane 2: ab224710 IP in U937 whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab224710 in U937 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 20 seconds.

  • Flow Cytometry - Anti-BAFF antibody [EPR22238] (ab224710)
    Flow Cytometry - Anti-BAFF antibody [EPR22238] (ab224710)

    Flow cytometric analysis of Daudi (human Burkitt's lymphoma cell line; left panel) and HL-60 (human promyelocytic leukemia cell line ;right panel) cell lines labeling BAFF with ab224710 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: Daudi. (PMID: 20951740)

    Gated on viable cells.

  • Immunocytochemistry/ Immunofluorescence - Anti-BAFF antibody [EPR22238] (ab224710)
    Immunocytochemistry/ Immunofluorescence - Anti-BAFF antibody [EPR22238] (ab224710)

    Immunofluorescent analysis of 100% methanol-fixed U937 (human histiocytic lymphoma cell line) cells labeling BAFF with ab224710 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on U937 cells.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    Confocal image showing membranous staining in U-937 cells.

    We don’t recommend PFA fixation, since the result shows some background staining in the nuclear.

    Negative control: HEK-293（PMID: 17393395.

  • Anti-BAFF antibody [EPR22238] (ab224710)
    Anti-BAFF antibody [EPR22238] (ab224710)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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