Human Thyroxine ELISA Kit (T4) (ab108661)
Key features and details
- Sensitivity: 0.4 µg/dl
- Range: 0.4 µg/dl - 25 µg/dl
- Sample type: Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Overview
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Product name
Human Thyroxine ELISA Kit (T4)
See all Thyroxine (T4) kits -
Detection method
Colorimetric -
Sample type
Serum -
Assay type
Competitive -
Sensitivity
= 0.4 µg/dl -
Range
0.4 µg/dl - 25 µg/dl -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
ab108661, Thyroxine (T4) Human ELISA Kit is intended for the quantitative determination of Total Thyroxine (T4) concentration in Human serum.
L-Thyroxine (T4) is a hormone that is synthesized and stored in the thyroid gland. Proteolytic cleavage of follicular thyroglobulin releases T4 into the bloodstream. Greater than 99% of Thyroxine (T4) is reversibly bound to three plasma proteins in blood - thyroxine binding globulin (TBG) binds 70%, thyroxine binding pre-albumin (TBPA) binds 20%, and albumin binds 10%. Approximately 0.03% of T4 is in the free, unbound state in blood at any one time.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests Enzyme Conjugate Concentrate (11x) 1 x 1.3ml Enzyme Conjugate Diluent 1 x 13ml Sheep anti-Tyroxine (T4) coated microtiter wells 1 x 96 units Stop Solution 1 x 11ml Thyroxine (T4) Reference Standard (0 µg/dl) 1 x 1ml Thyroxine (T4) Reference Standard (10 µg/dl) 1 x 1ml Thyroxine (T4) Reference Standard (15 µg/dl) 1 x 1ml Thyroxine (T4) Reference Standard (2 µg/dl) 1 x 1ml Thyroxine (T4) Reference Standard (25 µg/dl) 1 x 1ml Thyroxine (T4) Reference Standard (5 µg/dl) 1 x 1ml TMB Reagent 1 x 11ml -
Research areas
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Database links
- SwissProt: P05543 Human