Human BD-3 ELISA Kit (ab272471)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 14.3 pg/ml
- Range: 31.25 pg/ml - 2000 pg/ml
- Sample type: Cit plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human BD-3 ELISA Kit
See all BD-3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 2.4% Inter-assay Sample n Mean SD CV% Serum 3 3.8% -
Sample type
Serum, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
14.3 pg/ml -
Range
31.25 pg/ml - 2000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 97 92% - 100% Cit plasma 95 91% - 99% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human BD-3 ELISA kit (ab272471) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human BD-3 protein in serum and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human BD-3 with 14.3 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Notes
Defensins are cysteine rich cationic peptides known to exhibit strong antimicrobial and immune signaling activities. BD-3, encoded by the DEFB103A gene, is expressed in many epithelial cells and induced by cytokines or microbial stimuli. Studies shows that BD-3 may play an important role in the innate epithelial defense and wound healing.
-
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human BD-3 Capture Antibody 1 x 600µl 10X Human BD-3 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human BD-3 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Function
Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistent E.faecium. No significant hemolytic activity was observed. -
Tissue specificity
Highly expressed in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue. Low expression in salivary gland, bone marrow, colon, stomach, polyp and larynx. No expression in small intestine. -
Sequence similarities
Belongs to the beta-defensin family. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- BD 3
- BD-3
- BD3
see all -
Database links
- Entrez Gene: 414325 Human
- Entrez Gene: 55894 Human
- SwissProt: P81534 Human
- Unigene: 283082 Human
- Unigene: 637221 Human
Images
-
SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Example of human BD-3 standard curve in Sample Diluent NS.The BD-3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentration of spike BD-3 in human serum and plasma samples.The concentration of BD-3 were measured in duplicates, interpolated from the BD-3 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5% and plasma (citrate) 12.5%. The interpolated values are plotted (mean +/- SD, n=2).
-
Sandwich ELISA - Human BD-3 ELISA Kit (ab272471)To learn more about the advantages of recombinant antibodies see here.
