Human MSP ELISA Kit (MST1) (ab278126)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.118 ng/ml
- Range: 0.39 ng/ml - 25 ng/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human MSP ELISA Kit (MST1)
See all MSP/MST1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3% Inter-assay Sample n Mean SD CV% Serum 3 4% -
Sample type
Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.118 ng/ml -
Range
0.39 ng/ml - 25 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 85 80% - 91% Cell culture media 95 93% - 98% Hep Plasma 98 91% - 102% EDTA Plasma 82 72% - 87% Cit plasma 101 92% - 107% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human MSP ELISA kit (ab2778126) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human MSP protein in human serum. plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MSP with 118 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Macrophage-stimulating protein (MSP), also known as Hepatocyte Growth Factor-like Protein (HGFL) is a member of the HGF family of growth factors. MSP is secreted as inactive precursor. In response to injury, the MSP precursor is activated by proteolytic cleavage into a dimer of alpha and beta chains joined by a disulfide bond. The dimer Interacts (via beta chain) with MST1R.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human MSP (MST1) Capture Antibody 1 x 600µl 10X Human MSP (MST1) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human MSP (MST1) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 100ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Probably has no proteolytic activity, since crucial characteristic of serine proteases catalytic sites are not conserved. -
Sequence similarities
Belongs to the peptidase S1 family. Plasminogen subfamily.
Contains 4 kringle domains.
Contains 1 PAN domain.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsMay be cleaved after Arg-483, to yield two chains held together by disulfide bonds, or two separate polypeptides. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- D3F15S2
- DNF15S2
- Hepatocyte growth factor like protein
see all -
Database links
- Entrez Gene: 4485 Human
- Omim: 142408 Human
- SwissProt: P26927 Human
- Unigene: 349110 Human
- Unigene: 512587 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Recombinant Antibody benefitsTo learn more about the advantages of recombinant antibodies see here.
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Example of human MSP standard curve in Sample Diluent NS.The MSP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native MSP in human serum and plasma samples.The concentrations of MSP were measured in duplicates, interpolated from the MSP standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:1,000, plasma (citrate) 1:1,000, plasma (EDTA) 1:1,000 and plasma (heparin) 1:1,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MSP concentration was determined to be 24.7 µg/mL in neat serum, 24.7 µg/mL in neat plasma (citrate), 21.4 µg/mL in neat plasma (EDTA) and 23.3 µg/mL in neat plasma (heparin).
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MSP concentration was determined to be 19.6 µg/mL with a range of 13.6 – 26.7 µg/mL.
