Anti-Neutrophil Elastase antibody [NP57] (ab254178)
![Anti-Neutrophil Elastase antibody [NP57] (ab254178)](https://www.abcam.com/ps/products/254/ab254178/Images/ab254178-369250-AntiNeutrophil-Elastase-antibody-NP57-immunocytochemistry-u937-human.jpg "Anti-Neutrophil Elastase antibody [NP57] (ab254178)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [NP57] to Neutrophil Elastase
- Suitable for: ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-Neutrophil Elastase antibody [NP57]
See all Neutrophil Elastase primary antibodies -
Description
Mouse monoclonal [NP57] to Neutrophil Elastase -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Full length native protein (purified) within Human Neutrophil Elastase. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P08246 -
Positive control
- ICC/IF: U-937 cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
NP57 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Neutrophil Elastase antibody [NP57] (ab254178)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-937 (human histiocytic lymphoma monocyte) cells labeling Neutrophil Elastase with ab254178 at 11.16µg/ml, followed by Goat Anti-Mouse IgG (Alexa Fluor® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on U-937 cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab254178 at 1/50 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution.
-ve control 2: ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.Negative control: Jurkat（PMID: 22564522.
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![Anti-Neutrophil Elastase antibody [NP57] (ab254178)](https://www.abcam.com/ps/products/254/ab254178/Images/ab254178-2-benefits-of-recombinant-antibodies.png)
Anti-Neutrophil Elastase antibody [NP57] (ab254178)
