Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ADK antibody [EPR23166-143] - BSA and Azide free (ab267400)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23166-143] to ADK - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-ADK antibody [EPR23166-143] - BSA and Azide free
    See all ADK primary antibodies

  • Description

    Rabbit monoclonal [EPR23166-143] to ADK - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HepG2, Wild-type HAP1, Hepa1-6, PC-3, C6, RAW264.7, PC-12 and NIH/3T3. Mouse and rat liver lysates. IHC-P: Human tonsil, human liver, mouse cerebrum and rat kidney tissues. Flow Cyt: PC-3 cells.

  • General notes

    ab267400 is the carrier-free version of ab243636. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-ADK antibody [EPR23166-143] - BSA and Azide free (ab267400)
    Western blot - Anti-ADK antibody [EPR23166-143] - BSA and Azide free (ab267400)
    All lanes : Anti-ADK antibody [EPR23166-143] (ab243636) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : ADK knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 41 kDa
    Observed band size: 41 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab243636).

    Lanes 1- 2: Merged signal (red and green). Green - ab243636 observed at 41 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab243636 was shown to react with Adenosine kinase in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265646 (knockout cell lysate ab257343) was used. Wild-type HeLa and ADK knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab243636 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-ADK antibody [EPR23166-143] - BSA and Azide free (ab267400)
    Flow Cytometry - Anti-ADK antibody [EPR23166-143] - BSA and Azide free (ab267400)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized PC-3 (Human prostate adenocarcinoma epithelial cell) cells labeling ADK with ab243636 at 1\500 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243636).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADK antibody [EPR23166-143] - BSA and Azide free (ab267400)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADK antibody [EPR23166-143] - BSA and Azide free (ab267400)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling ADK with ab243636 at 1/1000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear and cytoplasmic staining on rat kidney is observed. The section was incubated with ab243636 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243636).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADK antibody [EPR23166-143] - BSA and Azide free (ab267400)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADK antibody [EPR23166-143] - BSA and Azide free (ab267400)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling ADK with ab243636 at 1/1000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear and cytoplasmic staining on mouse cerebrum is observed. The section was incubated with ab243636 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243636).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADK antibody [EPR23166-143] - BSA and Azide free (ab267400)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADK antibody [EPR23166-143] - BSA and Azide free (ab267400)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling ADK with ab243636 at 1/1000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear and cytoplasmic staining on human liver is observed. The section was incubated with ab243636 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243636).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADK antibody [EPR23166-143] - BSA and Azide free (ab267400)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADK antibody [EPR23166-143] - BSA and Azide free (ab267400)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling ADK with ab243636 at 1/1000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear and weak cytoplasmic staining on human tonsil is observed. The section was incubated with ab243636 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243636).

  • Anti-ADK antibody [EPR23166-143] - BSA and Azide free (ab267400)
    Anti-ADK antibody [EPR23166-143] - BSA and Azide free (ab267400)

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