Anti-AMPS antibody [EPR10747(B)] (ab154182)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10747(B)] to AMPS
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-AMPS antibody [EPR10747(B)]
See all AMPS primary antibodies -
Description
Rabbit monoclonal [EPR10747(B)] to AMPS -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human -
Immunogen
Synthetic peptide within Human AMPS. The exact sequence is proprietary. The antibody does not recognize human ADSL isoform 2.
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Positive control
- HeLa, Jurkat, HepG2 and HCT-116 cell lysates, Human thyroid gland carcinoma tissue, permeabilized HeLa cells
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General notes
Previously labelled as Adenylosuccinate Lyase.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10747(B) -
Isotype
IgG -
Research areas
Images
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Immunohistochemical analysis of paraffin-embedded human thyroid gland carcinoma tissue labeling AMPS with ab154182 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human normal brain tissue using ab154182 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Flow cytometric analysis of permeabilized HeLa cells with ab154182 at 1/100 dilution(red) or a rabbit IgG (negative) (green).
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All lanes : Anti-AMPS antibody [EPR10747(B)] (ab154182) at 1/1000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : Jurkat cell lysates
Lane 3 : HepG2 cell lysates
Lane 4 : HCT-116 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 55 kDa
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Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue using ab154182 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab154182 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab154182 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human normal liver tissue using ab154182 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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