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Signal Transduction Metabolism Energy Metabolism

Anti-AMPS antibody [EPR10747(B)] (ab154182)

Price and availability

291 484 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-AMPS antibody [EPR10747(B)] (ab154182)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10747(B)] to AMPS
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human

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Overview

  • Product name

    Anti-AMPS antibody [EPR10747(B)]
    See all AMPS primary antibodies
  • Description

    Rabbit monoclonal [EPR10747(B)] to AMPS
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human AMPS. The exact sequence is proprietary. The antibody does not recognize human ADSL isoform 2.

  • Positive control

    • HeLa, Jurkat, HepG2 and HCT-116 cell lysates, Human thyroid gland carcinoma tissue, permeabilized HeLa cells
  • General notes

    Previously labelled as Adenylosuccinate Lyase. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR10747(B)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] (ab154182)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] (ab154182)

    Immunohistochemical analysis of paraffin-embedded human thyroid gland carcinoma tissue labeling AMPS with ab154182 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] (ab154182)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] (ab154182)

    Immunohistochemical analysis of paraffin embedded Human normal brain tissue using ab154182 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-AMPS antibody [EPR10747(B)] (ab154182)
    Flow Cytometry - Anti-AMPS antibody [EPR10747(B)] (ab154182)
    Flow cytometric analysis of permeabilized HeLa cells with ab154182 at 1/100 dilution(red) or a rabbit IgG (negative) (green).
  • Western blot - Anti-AMPS antibody [EPR10747(B)] (ab154182)
    Western blot - Anti-AMPS antibody [EPR10747(B)] (ab154182)
    All lanes : Anti-AMPS antibody [EPR10747(B)] (ab154182) at 1/1000 dilution

    Lane 1 : HeLa cell lysates
    Lane 2 : Jurkat cell lysates
    Lane 3 : HepG2 cell lysates
    Lane 4 : HCT-116 cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 55 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] (ab154182)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] (ab154182)

    Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue using ab154182 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] (ab154182)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] (ab154182)

    Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab154182 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] (ab154182)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] (ab154182)

    Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab154182 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] (ab154182)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] (ab154182)

    Immunohistochemical analysis of paraffin embedded Human normal liver tissue using ab154182 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-AMPS antibody [EPR10747(B)] (ab154182)
    Anti-AMPS antibody [EPR10747(B)] (ab154182)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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