Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (ab109525)
![Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (ab109525)](https://www.abcam.com/ps/products/109/ab109525/Images/ab109525-122454-anti-proteasome-20s-alpha-2-antibody-epr5453-western-blot.jpg "Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (ab109525)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5453] to Proteasome 20S alpha 2/HC3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453]
See all Proteasome 20S alpha 2/HC3 primary antibodies -
Description
Rabbit monoclonal [EPR5453] to Proteasome 20S alpha 2/HC3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HepG2, 293T, HeLa or JAR cell lysate; Human brain tissue; HeLa cells
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General notes
This product was previously labelled as Proteasome 20S alpha 2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5453 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (ab109525)All lanes : Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (ab109525) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : JAR cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 25 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (ab109525)](https://www.abcam.com/ps/products/109/ab109525/Images/ab109525-122453-anti-proteasome-20s-alpha-2-antibody-epr5453-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (ab109525)ab109525, at a 1/250 dilution, staining Human Proteasome 20S alpha 2/HC3 in brain tissue, using Immunohistochemistry, (Formalin/PFA-fixed paraffin-embedded).
Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (ab109525)](https://www.abcam.com/ps/products/109/ab109525/Images/ab109525-122452-anti-proteasome-20s-alpha-2-antibody-epr5453-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (ab109525)ab109525, at 1/50 dilution, staining Proteasome 20S alpha 2/HC3 in HeLa cells by Immunofluorescence.
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![Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (ab109525)](https://www.abcam.com/ps/products/109/ab109525/Images/ab109525-4-benefits-of-recombinant-antibodies.png)
Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (ab109525)
